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Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

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If the Expression is a delimiter box is not checked, your regular expression must<br />

completely define each Assembly H<strong>and</strong>le <strong>and</strong> Name Delimiter in your sequence name.<br />

The regular expression you write will not work unless it describes the entire name of your<br />

sequences. When you type the regular expression into the text field, it should be in the<br />

form:<br />

(Assembly H<strong>and</strong>le1)Name Delimiter(Assembly H<strong>and</strong>le2)<br />

In the example in Figure 10-3 below, a regular expression is used to combine the first<br />

two parts of the sequence name, Origin <strong>and</strong> Clone, to create a new Assembly H<strong>and</strong>le,<br />

Origin&Clone. The Preview button displays the results of your regular expression. If<br />

you are satisfied, click on the OK button.<br />

Figure 10-3 Advanced Expression for Name Parsing dialog window<br />

For more details on regular expressions, see Appendix 24 “Advanced Expression” <strong>and</strong><br />

the tutorials in the Tutorials folder.<br />

Setting your Assembly H<strong>and</strong>le Names<br />

Starting in the Project Window, click on the Assembly Parameters button. At the<br />

bottom of the Assembly Parameters window, click on the Name Settings… button.<br />

The Assemble by Name Settings window appears as in Figure 10-4.<br />

You can describe up to eight Assembly H<strong>and</strong>les of which only one is active at any one<br />

time.<br />

For each Assembly H<strong>and</strong>le, you can use the default name <strong>Sequencher</strong> automatically<br />

gives your contigs or you can type a descriptive title into the text box. Click on the radio<br />

SEQUENCHER <strong>4.8</strong> <strong>User</strong> <strong>Manual</strong> for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.<br />

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