Research Report 2010 - MDC

Development of an MRM method for quantification of peptides.Starting from the initial MS/MS spectrum of the peptide of interestthe MRM-transitions are constructed. Elution profile of different peptidesdeducted from MRM measurements, which lead to the quantificationof the peptide of interest.translational modifications. For the understanding ofthe molecular mechanisms, which regulate these proteins,it is necessary to gain insight into the differentpost-translational modifications of proteins. The corefacility actively pursues the development of new methodsfor the identification of modification sites by ubiquitin-likeproteins. For the identification of phosphorylationsites the core facility now provides specializedmethods, e.g. phospho-ion scan with polarity switchingfor improved sensitivity.Selected PulicationsDittmar, G. and Selbach, M. Novel insights into proteomic technologiesand their clinical perspective. Genome Med (2009) vol. 1 (5) pp. 53.Guenther, U.-P., Handoko, L., Varon, R., Stephani, U., Chang-Yong, T.,Mendell, J., Lützkendorf, S., Hübner, C., von Au, K., Jablonka, S., Dittmar, G.,Heinemann, U., Schütz, A. and Schülke, M. Clinical variability in distalspinal muscular atrophy type 1 (DSMA1): determination of steady-stateIGHMBP2 protein levels in five patients with infantile and juveniledisease. J Mol Med (2009) vol. 87 (1) pp. 31-41.Schwanhäusser, B., Gossen, M., Dittmar, G. and Selbach, M. Globalanalysis of cellular protein translation by pulsed SILAC. Proteomics(2009) vol. 9 (1) pp. 205-9.Technology Platforms 223