Research Report 2010 - MDC

Figure 3. Proof of principle: FACS sorting of a mixed C. elegans embryo population (eFACS) routinely yields tens of thousands of almost perfectlyclean one-cell embryo population (Stoeckius & Maaskola et al, Nature Methods 2009). A transgenic strain that expresses GFP mosthighly in the one cell embryo is used. Embryos are fixed with Methanol, and the GFP positive population marked in green (a) is resorted (b). Arandom sample of 96 embryos from the resorted population consists to at least 98% of one cell embryos, as shown by microscopic analysis(c) and also confirmed by PCR markers (d).stem cells. These projects involve, in part, collaborationswith the Sanchez lab (U. of Utah), S. Kempa (MDC), W.Chen (MDC) and others.Selected PublicationsKrek, A, Gruen, D, Poy, MN, Wolf R, Rosenberg L, Epstein EJ, MacMenamin P,da Piedade I, Gunsalus KC, Stoffel M, and Rajewsky N (2005).Combinatorial microRNA target predictions. Nature Genetics 37, 495-500Chen, K and Rajewsky, N. (2006). Natural selection on human microRNAbinding sites inferred from SNP data. Nature Genetics 38, 1452-1456.Chen, K and Rajewsky, N. (2007). The evolution of gene regulation bytranscription factors and microRNAs.. Nature Reviews Genetics 8, 93-103.Selbach M; Schwanhaeusser B; Thierfelder N; Fang Z; Khanin R; andRajewsky N (2008). Widespread changes in protein synthesis induced bymicroRNAs. Nature 455, 58-63Friedlaender MR; Adamidi C; Han T; Lebedeva S; Isenbarger TA; Hirst M;Marra M; Nusbaum C; Lee WL; Jenkin JC; Alvarado AS; Kim JK; andRajewsky N. (2009). High-resolution profiling and discovery of planariansmall RNAs. PNAS 28, 11546-11551Cardiovascular and Metabolic Disease Research 29