Research Report 2010 - MDC

Examples fort the use of EM methods for phenotyping and immunolabelinga. cell culture of human embryonic stem cells, line SA 002, embeddingin Epon as for EM, semithin section (1µm), light microscopy,bar = 50µma spontaneous differentiation of cells towards high prismatic,epithelial-like structures becomes visible (arrowhead)d. saphenous nerve of a mole rat, embedding in Epon, ultrathinsection, bar = 2µmthe number of myelinated and unmyelinated (arrowheads) axonscan be counted, and g-ratios can be calculated from myelin andaxon perimeters (coulored lines)b.cell culture of neonatal rat cardiomyocytes, embedding in Epon,ultrathin section, bar = 1µmcell components like growing myofibrils and cell-cell-contacts(arrowhead) are detectablee. limb muscle of a mouse embryo, embedding in Epon, ultrathinsection, bar = 500 nmidentification of satellite cells (sc) among muscle cells (mc) and othercell types is done finally by electron microscopy, as depicted by thebasal membrane covering both cell types (arrowheads)c. hippocampus of an adult mouse, ultrathin cryosection accordingto Tokuyasu, bar = 250 nmimmunolabeling of amyloid precursor protein (APP) in Golgi fields,12 nm colloidal goldheart, we firstly localized the soluble epoxide hydroxylase(sEH), an enzyme with putative importance to preventheart failure (collaboration with W.-H. Schunck).Selected publicationsWetzel, C, Hu, J, Riethmacher, D, Benckendorff, A, Harder, L, Eilers, A,Moshourab, R, Kozlenkov, A, Labuz, D, Caspani, O, Erdmann, B, Machelska,H, Heppenstall, PA, Lewin, GR. (2007). A stomatin-domain proteinessential for touch sensation in the mouse. Nature 445, 206-209.Vasyutina, E, Lenhard, DC, Wende, H, Erdmann, B, Epstein, JA, Birchmeier,C. (2007). RBP-J (Rbpsuh) is essential to maintain muscle progenitor cellsand to generate satellite cells. PNAS 104, 4443-4448Rohe, M, Carlo, A-S, Breyhan, H, Sporbert, A, Militz, D, Schmidt, V, Wozny, C,Harmeier, A, Erdmann, B, Bales, KR, Wolf, S, Kempermann, G, Paul, SM,Schmitz, D, Bayer, TA, Willnow, TE, Andersen, OM. (2008) Sortilin-relatedreceptor with A-type repeats (SORLA) affects the amyloid precursorprotein-dependent stimulation of ERK signaling and adult neurogenesis.J Biol. Chem. 283, 14826-14834König, O, Rüttiger, L, Müller, M, Zimmermann, U, Erdmann, B, Kalbacher, H,Gross, M, Knipper, M. (2008) Estrogen and the inner ear: Megalinknockout mice suffer progressive hearing loss. FASEB J. 22, 410-417Gramlich, M, Michely, B, Krohne, C, Heuser, A, Erdmann, B, Klaassen, S,Hudson, B, Magarin, M, Kirchner, F, Todiras, M, Granzier, H, Labeit, S,Thierfelder, L, Gerull, B. (2009, 47, 352-358) Stress-induced dilatedcardiomyopathy in a knock-in mouse model mimicking human titinbaseddisease. J Mol Cell Cardiol 47, 352-358Technology Platforms 229