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Revision of Passiflora Subgenus Decaloba ... - Passion Flowers

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131<br />

the molecular study was not feasible due to limited sample availability and<br />

expense <strong>of</strong> analysis. There were also several polymorphic sites evident in the<br />

ITS sequences for several <strong>of</strong> the species within supersection Cieca, further<br />

increasing the need for more infraspecific sampling. Most importantly, the<br />

complex evolutionary history (involving hybridization and polyploidy) <strong>of</strong> several <strong>of</strong><br />

the entities within the group has undoubtedly had a significant effect on both data<br />

sets, increasing the amount <strong>of</strong> incongruity in the analyses.<br />

In an attempt to overcome the difference in sample sizes between the<br />

morphological and molecular data sets, additional cladistic analyses were<br />

undertaken in which the operational taxonomic units were reduced to those for<br />

which both molecular and morphological data were available. Separate analyses<br />

<strong>of</strong> the reduced morphological and molecular data sets resulted in the production<br />

<strong>of</strong> four equally parsimonious trees that were 224 steps long and 1,638 equally<br />

parsimonious trees that were 332 steps in length, respectively. The consensus<br />

trees from both <strong>of</strong> these analyses were compared and were conspicuously<br />

incongruent in their topologies. In addition, there were significant changes in the<br />

resulting phylogenies, as compared to the original analyses, that were likely due<br />

to decreased (and likely inadequate) taxon sampling. I have chosen not to<br />

combine my morphological and molecular data sets because <strong>of</strong> this<br />

incongruence and the knowledge that complex biological processes are likely<br />

influencing the pattern <strong>of</strong> diversity within <strong>Passiflora</strong> supersection Cieca.<br />

On a more positive note, the phylogenetic hypotheses based on the<br />

morphological and molecular data sets for supersection Cieca agree in several

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