Revision of Passiflora Subgenus Decaloba ... - Passion Flowers

428
monophyly of P. viridiflora (82% bootstrap). Passiflora itzenis and P. xiikzodz
form a clade with 100% bootstrap support; however, the clones from an
individual of P. xiikzodz do not form a monophyletic group. The sister
relationship of P. sexocellata and P. coriacea is strongly supported (94%
bootstrap). Passiflora obtusifolia is sister (82% bootstrap) to a clade containing
most (but not all) of the clones of two individuals of P. suberosa ssp. suberosa
from Puerto Rico and St. Croix. Passiflora lancifolia is most closely related to a
clone of P. pallida from Jamaica and P. suberosa s.l.
Of particular interest are the clones of the various entities of P. xiikzodz, P.
viridiflora, P. suberosa, and P. pallida (Fig. A.2-A.3). Sequences cloned from a
single individual of Passiflora xiikzodz from México are found in two different
clades. Two clones ("a" and "c") constitute a moderately supported clade (74%
bootstrap), and the other two ("b" and "d") are sister to P. itzensis. These data
show that there are at least four copies of GBSSI present in P. xiikzodz, a
putative diploid (Snow and MacDougal, 1993). Clones from an individual of P.
viridiflora constitute a clade, but at least four copies of GBSSI are present in that
species. There are also at least three copies of this gene in individuals of P.
suberosa ssp. suberosa from Puerto Rico and St. Croix. An individual of
Passiflora suberosa ssp. litoralis from Taxco in the state of Guerrero, México
possesses at least five different copies of waxy. Passiflora pallida from Jamaica
also has at least five copies, and P. pallida from Puerto Rico possesses four.
The topologies of the gene trees resulting from the separate analyses of the
molecular ITS data (see Figs. 4.1-4.4) and the GBSSI data (see Figs. A.2-A.3)