Revision of Passiflora Subgenus Decaloba ... - Passion Flowers

CHAPTER 4
MOLECULAR ANALYSES
The cladistic analysis (PAUP* 4.0b10) of the molecular ITS-1 and ITS-2 data
resulted in the generation of three equally parsimonious trees (Figs. 4.1-4.3) of
590 steps, a consistency index (CI) of 0.636, and a retention index (RI) of 0.837.
The topologies presented in these trees are all quite similar, with only minor
rearrangements occurring within the P. pallida and P. suberosa clades. The
strict consensus tree is presented in Fig. 4.4.
Supersections Decaloba and Multiflora appear to be monophyletic, with
bootstrap values of 92% and 100%, respectively. The two taxa sampled from
supersection Decaloba section Xerogona, P. citrina J. M. MacDougal and P.
rubra L., form a monophyletic group (100% bootstrap). Passiflora penduliflora
Bertero ex DC. and P. perfoliata L. are also monophyletic in this analysis (100%
bootstrap). The species sampled from supersection Multiflora (P. inca P. Jørg.,
P. lobbii Mast. ssp. ayacuchensis Skrabal and Weigend, and P. podlechii Skrabal
and Weigend) are monophyletic. Passiflora lobata (Killip) Hutch. ex J. M.
MacDougal (supersection Bryonioides) forms a polytomy at the base of the tree
with supersection Multiflora.
The monophyly of supersection Cieca is strongly supported (100% bootstrap).
There is also evidence for the monophyly of P. pallida (95% bootstrap), P.
lancifolia (87% bootstrap), P. tenuiloba (100% bootstrap), P. sexocellata (93%
bootstrap), P. viridiflora (96% bootstrap), P. juliana (100% bootstrap), P.
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