Revision of Passiflora Subgenus Decaloba ... - Passion Flowers

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repository of foreign place-name decisions approved by the United States Board
on Geographic Names (US BGN). A secondary source for international localities
was the Worldwide Directory of Cities and Towns (http://www.calle.com/world).
The coordinate system for data served by the GNIS, GNS and the Worldwide
Directory of Cities and Towns is WGS84.
Principal components analyses (PCA) were produced using the computer
program Multi-Variate Statistical Package (MVSP) 3.13d (Kovach Computing
Services, Anglesey, Wales, UK). All measured quantitative characters for
specimens in the P. suberosa and P. coriacea complexes were used in the
analyses. Only those characters and specimens for which there was an
abundance of missing data were deleted from the analyses. The mean values of
measurements were used for each specimen. Box plots were produced using
the computer program SPSS for Windows Release 11.5 (SPSS, Inc., Chicago,
Illinois, USA). Neighbor joining trees were produced in PAUP* Version 4.0b10
for Macintosh (Sinaeur, Sunderland, Massachusetts, USA). Morphological
characters were also analyzed cladistically, and cladistic methods are discussed
under the heading "Phylogenetic Search Strategies.”
Molecular Data Set
Total genomic DNA was extracted from fresh, heat dried, or silica dried leaves
or flowers utilizing the CTAB method of Doyle and Doyle, scaled down to 1.0 ml
extraction volumes (Doyle & Doyle, 1987). DNA was precipitated for
approximately one to eight hours at -20ºC with 0.65 volume of isopropanol,
centrifuged, washed twice with 70% ethyl alcohol, and dried. The pellet was
resuspended in 75µl of Tris-EDTA buffer and stored at -20ºC.