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Vol. 53 - Alaska Resources Library and Information Services

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obtain sufficient weights all the stomachs at a station were pooled into<br />

one sample. Dividing the dry weights of individual prey items by the<br />

total dry weight of all items <strong>and</strong> multiplying by 100 gave dietary<br />

composition as a percentage of total dry weight. Because floc <strong>and</strong> s<strong>and</strong><br />

proved a substantial proportion of the dry weight, calculations were done<br />

both including <strong>and</strong> excluding floc <strong>and</strong> s<strong>and</strong>.<br />

CALORIC INTAKE<br />

To determine caloric intake the dry weights for each item needed to be<br />

converted to caloric equivalents. However, simple conversion of dry<br />

weights of stomach contents to calories was not possible without an<br />

estimate of the intake of soft tissue. Caloric values are determined on<br />

the soft tissue of marine invertebrates after the shell or other hard<br />

parts have been removed. Because the dry weights of prey items were<br />

determined almost exclusively by weighing hard tissue, such as shell,<br />

estimating the dry weight of soft tissue ingested from the dry weights of<br />

hard parts was necessary before conversion to calories was possible. For<br />

a number of potential prey items collected from the Bering Sea, the soft<br />

tissue was separated from the hard tissue, <strong>and</strong> both dried at 60 C in tared<br />

containers to constant weight. Soft tissue/hard tissue conversion factors<br />

were calculated by dividing the dry weight of the soft tissue by the dry<br />

weight of the hard tissue. Conversion factors for prey items not<br />

determined experimentally were estimated by their similarity to those<br />

items for which data exists. Floc was assumed to be essentially soft<br />

tissue <strong>and</strong> assigned a soft tissue/hard tissue ratio of 1.000.<br />

To obtain dietary composition on the basis of soft tissue dry weights,<br />

the dry weight for each prey item was multiplied by its soft tissue/hard<br />

tissue ratio, the resulting soft tissue dry weights summed to obtain a<br />

total, <strong>and</strong> the percentage of the diet calculated based on that total.<br />

Correction for gut residence time followed the same procedure described<br />

above for dietary composition as percentage of all occurrences.<br />

To obtain the energetic contribution to the diet made by each prey<br />

item, the corrected percentage of diet on the basis of total soft tissue<br />

dry weight was multiplied by the daily ration (mg/g crab wet weight) to<br />

yield the soft tissue dry weight for each item. Then these soft tissue<br />

dry weights were multiplied by the calcric values (calcries/mg dry weight)<br />

to obtain the calcries ingested from each prey item. Caloric values came<br />

either from the literature or from new determinations done on specimens<br />

collected from the Bering Sea. These new determinations of caloric value<br />

were done by st<strong>and</strong>ard bomb calcrimetry with benzoic acid st<strong>and</strong>ards at<br />

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