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Vol. 53 - Alaska Resources Library and Information Services

Vol. 53 - Alaska Resources Library and Information Services

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clams from Bay 9 in the second post-spill sample was the only value which was<br />

significantly different from the corresponding value in clams from the reference bay.<br />

Concentrations of total other carbohydrates, which consist of trehalose <strong>and</strong> other nonglycogen<br />

oligosaccharides <strong>and</strong> polysaccharides which yield glucose upon hydrolysis, were<br />

highly variable <strong>and</strong> no obvious trends among samples from different bays or sampling<br />

times were apparent.<br />

Concentrations of total lipids in clams from Bays 7, 10 <strong>and</strong> 11<br />

dropped<br />

between the pre-spill <strong>and</strong> first post-spill samples <strong>and</strong> then returned to pre-spill or higher<br />

values by the time of the second post-spill sampling.<br />

In clams from Bay 9, total lipid<br />

concentration increased between pre-spill <strong>and</strong> first post-spill <strong>and</strong> then dropped to the prespill<br />

range by the time of the second post-spill sampling.<br />

The degree of contamination of Mya truncata with petroleum hydrocarbons<br />

varied greatly within each bay depending on the station at which clams were collected<br />

(see Section 2.).<br />

Data were available by station for petroleum hydrocarbon burden <strong>and</strong><br />

tissue glucose <strong>and</strong> glycogen concentration in Mya truncata from the second post-spill<br />

collection (Table 4.2). Mean body burdens of petroleum hydrocarbons in clams from 30<br />

stations in 4 bays ranged from 35 to 238 µg/g (ppm). However, there was no relationship<br />

between body burden of petroleum hydrocarbons <strong>and</strong> concentrations of glucose <strong>and</strong><br />

glycogen in the tissues of clams. In Bays 9 <strong>and</strong> 10, clams were collected at both the 3-<br />

meter <strong>and</strong> 7-meter isobaths. There were no statistically significant differences between<br />

clams from the two depths in concentration in the tissues of petroleum, glucose, or<br />

glycogen.<br />

Fourteen different free amino acids were identified <strong>and</strong> quantified in the<br />

adductor muscles of Mya truncata from the four bays. The mean concentration of total<br />

free amino acids ranged from 12.45 to 23.25 µM/mg wet weight in clams from different<br />

bays at different sampling times (Tables 4.3, 4.4, <strong>and</strong> 4.5). In clams from the two bays<br />

receiving dispersed oil (Bays 9 <strong>and</strong> 10), the mean concentration of tissue total free amino<br />

acids dropped between the pre-spill <strong>and</strong> first post-spill samples <strong>and</strong> then rose again in the<br />

second post-spill samples.<br />

The opposite trend was observed in clams from the bay<br />

receiving oil alone (Bay 11), while tissue total free amino acid concentrations in clams<br />

from the reference bay (Bay 7) remained relatively constant (range 15.37-17.65 µM/mg).<br />

83

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