CinePlex v3 Manual - Plexon Inc

stamped with corresponding MAP times. For more information on this capability,
see “Digital Video Recording” on page 55.
2 Position Tracking: If the Tracking option is licensed, CinePlex Studio provides
the ability to track the position of subject(s) in the video images, in real
time, and deliver the tracked position coordinates to a Plexon NDAQ. Tracking
can also be used with existing pre-recorded files. When the Tracking
option is enabled, there are three different modes of operation:
a
b
Object Contour Mode: In this mode, CinePlex Studio uses a setup
procedure to color contrast and threshold the image until the subject
being tracked is all white and the background is all black. For each
frame, CinePlex Studio computes the centroid of the white pixels to
obtain the tracking coordinates. This mode of tracking operation is
described in “Object Contour Mode Tracking” on page 75.
LED Tracking Mode: In this mode, CinePlex Studio tracks up to five
light emitting diodes (LED) on the subject or subjects being tracked. To
improve tracking, LED Tracking Mode applies filters that remove the
halo surrounding bright LEDs. This mode of tracking operation is
described in “LED Mode Tracking” on page 82.
c Reflective Colors Tracking Mode: In this mode, CinePlex Studio
tracks up to three colors (tape or paint, for example) on the subject or
subjects being tracked. This mode of operation is described in “Reflective
Colors Mode Tracking” on page 85.
Guidelines for choosing which tracking mode to use for a particular experiment
are discussed in “CinePlex Studio Tracking Pane (if available)” on page 72.
Note:
The user may configure CinePlex Studio to capture video only, or to track positions
only, or to both capture video and track positions simultaneously.
For either function, the user must mount the CinePlex Studio video camera(s) a
short distance away from an appropriately designed experimental arena. Adjust
the camera(s) to have the appropriate field of view (see Lens Selection on
page C-3) so that the entire experimental arena is visible on the video images.
Arena functions are enabled when Tracking is available. Using the tools provided,
an outline of the experimental arena can be drawn over the video image(s).
Once drawn, Studio ignores objects outside of the arena when processing the
images. This results in lower CPU usage and reduces false object detections due
to reflections and shadows. It also allows the experimenter to exclude areas of the
image that have no relevance to the experiment. Arena tools allow drawing circles,
ellipses, rectangles, and freehand objects. Arena logical operations (AND,
OR, NOT, XOR) allow combining multiple arena shapes to produce a single
complex arena shape.
3 Behavior Monitoring: If the Behavior option is available, tracked objects
representing experimental subjects can cause logical and digital events by tra-
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