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CinePlex v3 Manual - Plexon Inc

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2<br />

2.9 <strong>CinePlex</strong> Studio Tracking Pane (if available)<br />

As described in Chapter 1, the <strong>CinePlex</strong> Studio Tracking option can generate<br />

tracking coordinates in one of three modes: Object Contour, LED, or Reflective<br />

Colors. The Tracking tab is blank until a tracking mode has been selected.<br />

<strong>CinePlex</strong> Studio uses Tracking Windows and Arena Definition to track objects<br />

and reduce unwanted parasite objects. These elements are explained below.<br />

2.9.1 Tracking Window<br />

Tracking windows are used to reduce problems with unwanted parasite objects<br />

(e.g. reflections) and to reduce the processor load by restricting analysis to a<br />

small portion of the video image.<br />

The size of the tracking window is set automatically to cover the whole object.<br />

Each object (whole animal, LED, or marker) has its own tracking window. The<br />

tracking window is repositioned automatically. A history of an object’s movement<br />

is used to predict its next position. If the object disappears because of occlusion<br />

by a cable or other means, its tracking window is moved according to the<br />

speed and movement direction in its most recently detected frames. If the system<br />

is tracking multiple LEDs or markers in single animal mode, the system will also<br />

use the positions of the found LEDs or markers to predict the position of the one<br />

that disappeared. If the object is not found after a certain number of frames, the<br />

size of the tracking window will be increased. If the object is not found within<br />

two seconds, the system switches to search in the whole frame (possibly<br />

restricted by an arena - see below).<br />

2.9.2 Arena Definition in the Areas Tab<br />

The Arena Definition is also used to reduce problems with unwanted parasite<br />

objects outside of the working area. If a reflection of other unwanted object<br />

appears outside the arena, it will not be detected by <strong>CinePlex</strong> Studio.<br />

72 <strong>CinePlex</strong>

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