CinePlex v3 Manual - Plexon Inc

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1 versing zones within the arena, sequences of zones, matching angle targets for head vectors, and meeting speed targets. In addition users with two cameras can define combinations of events that, in turn, can cause other digital events. a b c d Zones: A zone is a defined portion of the arena that has significance in an experiment. Zones that do not move with respect to the image or the arena are referred to as static zones. For example, a static zone could be an area the animal should avoid to receive a reward or it could be an area the animal must traverse to receive the reward. It could even be one of the zones in a sequence of zones the animal should traverse. CinePlex Studio provides tools to allow the user to draw zone outlines on top of the video image. These tools operate in the same way as the arena tools, allowing complex shapes and logical operations. CinePlex Studio also allows the definition of dynamic zones that are circular areas around the center of gravity of a tracked animal or color. Dynamic zones are treated the same logically as static zones. Many zones can be defined simultaneously. Sequences: An ordered list of zones in an arena (called a sequence) can be defined. Many different sequences can be defined. Events: Logical events can be defined so that, when an animal enters or leaves a zone, or completes a sequence, the event becomes true. Many events can be specified. Their current states are dynamically displayed in the Event Statistics pane. Combination Events: A combination event is an event that becomes true when two or more other events are true. The events used in a combination event can be generated from one or both cameras. This allows events to be generated by simultaneous occurrences in two different images, if desired. e Digital Outputs: The experimenter can specify that a digital output be generated when any event occurs. Up to 24 different digital outputs can be specified, any of which can be pulsed, high or low when active. CinePlex Studio can be started from an icon or the Programs Menu. For a full description of the user interface elements, “Main Window” on page 120. 1.1.5 About CinePlex Editor CinePlex Editor is a software tool that enables the user to view and edit video and neural data files. With CinePlex Editor, the user may view time-correlated video, neural, and positional data. The user may also use CinePlex Editor to add and edit annotations and perform other operations on the data, such as artifact removal. CinePlex Editor records user actions to its own separate CinePlex project file, so the user may continue annotation and editing work over several sessions. When the the markup process is completed, the user may save the results and export the data for further analysis. For a complete list of CinePlex 6 CinePlex
Editor features, see “Offline extraction of selected static and dynamic data from CinePlex video files” on page 3. CAUTION Upgrade Notice If the system has been upgraded to CinePlex 3.0, CinePlex Editor should also be updated. The upgraded CinePlex Editor is backwards compatible and will also read AVI files created by CinePlex Capture 1.0, 1.1, 1.2, 1.4, and 2.0. Version 3.0 7
Page 1 and 2: Neurotechnology Research Systems Us
Page 3 and 4: CinePlex Video, Capture, Tracking,
Page 5 and 6: Plexon Inc Publication History iii
Page 7 and 8: Plexon Inc Contents v Publication H
Page 9 and 10: Plexon Inc CinePlex vii File Toolba
Page 11 and 12: Plexon Inc CinePlex ix Appendix B P
Page 13 and 14: Plexon Inc Chapter 1 CinePlex Syste
Page 15 and 16: — All-digital imaging with one or
Page 17: stamped with corresponding MAP time
Page 21 and 22: 1.3 CinePlex System Hardware and So
Page 23 and 24: The Quad MAP Controller (QMC) can c
Page 25 and 26: 1.4 Data Analysis After recording,
Page 27 and 28: Plexon Inc Chapter 2 Using CinePlex
Page 29 and 30: 2.2 Designing the Experiment The pr
Page 31 and 32: 2.4 Performing an Operational Test
Page 33 and 34: 4 Start Sort Client program and ver
Page 35 and 36: 7 Start CinePlex Studio program. Th
Page 37 and 38: will not be able to distinguish the
Page 39 and 40: 2.5.4.2.3 LEDs in Light with Reflec
Page 41 and 42: the object and the yellow rectangle
Page 43 and 44: 2.5.4.3.7 Multicolored Animal. The
Page 45 and 46: Selecting the Tracking Mode 1 Selec
Page 47 and 48: 3 Check the color to track (by clic
Page 49 and 50: that sizes, positions and velocitie
Page 51 and 52: d e Measure its longest dimension i
Page 53 and 54: In the above image note that the si
Page 55 and 56: d e Measure the feature’s horizon
Page 57 and 58: i If the current color in the box n
Page 59 and 60: n Go to the slider next to the Vert
Page 61 and 62: 6 Repeat these steps for each addit
Page 63 and 64: Defining Logical Events Logical eve
Page 65 and 66: 1 Click the Add new combination of
Page 67 and 68: 2.6 Executing the Experiment Plan T
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also use a variety of commercial so
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When the Start button is clicked, S
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Note: If Studio is armed on one day
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settings and use the file mode to d
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2 To set or change the folder locat
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3 Open a second Windows Explorer an
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2.7 Global Configuration Pane The G
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2.8 Source Pane The Source Pane def
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The Arena Definition is also used t
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• In Reflective Colors mode, pixe
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Configuring CinePlex Studio to trac
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emove progressively larger features
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The illustration below is taken aft
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Configuring CinePlex Studio to trac
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2 In the Video window a square area
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If Pure Colors is not selected, the
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Increase the threshold to reduce th
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2.10 Operating CinePlex Studio with
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To verify CinePlex Studio data acqu
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3 In the Control View, click Connec
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2.10.1.1 Control View VTViewer disp
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2.10.1.3 VTViewer Options The Optio
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2.11 CinePlex Studio Offline Mode 2
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• Sequences Pane(s) (if behavior
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2.13 CinePlex Studio Test Mode Test
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2.14 Operation with the Quad MAP Co
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6 Connect a push button switch (09-
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Version 3.0 111
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15 On each CPX, launch and arm CPX.
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2.15 Using the Extractor This chapt
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illustrates. Note that the Marker 4
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Plexon Inc Chapter 3 CinePlex Studi
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3.2.1 Static Configuration Pane The
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3.2.1.2.1 Camera Group. The Camera
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• Brightness - this setting speci
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3.2.1.3 Tracking Tabs One of three
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3.2.1.4.1 Object Contour Tracker Gr
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• Object Transparency - This sett
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• Cent. Grav. Size - This setting
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• Mrk1Threshold, Mrk2Threshold, M
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3.2.2 Dynamic Configuration Pane Th
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• Subtract shapes - - this button
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• Add zone to selected sequence -
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• Delete selected tracking event
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3.2.2.4.1 Toolbar. The toolbar cont
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Note: The effects of the Image Qual
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— Name - this value depends on wh
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3.3 Menus The CinePlex Studio File
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3.3.3 DVR Menu 3.3.3.1 Arm Clicking
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3.4 Toolbars The File, DVR and Trac
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ow,time,x1,y1,x2,y2,x3,y3,x4,y4,x5,
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3.6.3 Source Settings The Source se
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Plexon Inc Chapter 4 Using CinePlex
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4.1.3 Changing and Saving Project F
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4.2.2 Setting Up a New Project This
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4.2.3 Understanding the CinePlex Ed
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4.2.4 Defining a New Marker This pr
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Markers window in the Name column a
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3 In the Format area, accept the de
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4.4 Customizing the User Interface
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In the Input window, select an inpu
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matically advances the video by a p
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4.5.3 Editing Markers and Marker Oc
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Selected Marker Occurrence, or clic
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3 Select entries in the Marker 1 an
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is defined and selected. A more sop
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corner of the image. If the video r
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Finding marker occurrences with a b
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2 Choose a marker from the Marker l
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Interpolating missing occurrences 1
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lowing illustration idx indicates t
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6 The user may set the columns for
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4.6 CinePlex Editor How-Tos This se
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However, do not expand the range so
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name and click Export! The Export C
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7 Repeat Step 6 until all the coord
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Plexon Inc Chapter 5 CinePlex Edito
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5.2 Main Window This section descri
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Show Complete Tail feature is usefu
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The color drawn at point p in the t
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— By pressing the PAGE UP or PAGE
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• Automatically Adjust Label Area
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Note: — Type - In the Type area,
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ox and select a different key. When
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Note: By default, if the Show check
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— Data Type: - This item contains
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5.2.8.1 Function The Messages windo
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• Add, Edit, and Delete Marker Oc
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• Reset Automatic Range Adjustmen
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5.3 Menus Menus provide access to C
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— Video Data Input File (AVI) - T
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After verifying that the correct fi
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• Which Markers - The default val
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• File - Type an export file name
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5.3.2 Edit Menu The following illus
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Note: The Edit Marker dialog box ma
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• Marker - This box shows the mar
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5.3.4.7 Step Forward This button ad
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5.3.6 Find Menu The following illus
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• Generate Selected - This button
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• Generate All - This button gene
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— Zone Interval • Operation to
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5.3.7.5 Snap-To Marker Occurrences
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— max - the largest time differen
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5.3.7.8.1 General Tab. • Initial
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5.3.7.8.3 Latency Correction Tab. F
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5.3.7.8.5 Audio Tab. • Available
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— Background - This is the backgr
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5.4 Toolbars CinePlex Editor includ
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5.5 How CinePlex Editor Creates Ini
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then load a neural data file that c
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Marker Type Abbr Information Stored
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From the Marker Occurrences window
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Note that applying this correction
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Snap-To Marker Occurrences tool des
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5.10 Analyzing CinePlex Studio data
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Plexon Inc Appendix A Installation
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Procedure for MAP/CPX Computer Hard
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A.2 Installation - Software This se
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3 The End User License Agreement wi
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7 The Select Program Manager Group
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11 The following window appears twi
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14 Click Next on the window below.
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18 The CinePlex installation has no
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3 The End User License Agreement wi
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7 The Select Program Manager Group
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11 Next, the Sentinel Key Drivers a
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15 Click Finish on the window below
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2 The Welcome window appears. Press
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6 The Select Components window appe
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10 Nothing else will be installed,
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3 After step 6 continue the procedu
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Note: to remote upgrades. If a remo
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Plexon Inc Appendix B Problem Resol
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toggle switch above these connector
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the CinePlex software will be requi
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1 Remove the TIM card and check tha
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1 Stop running all unnecessary appl
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B.3 CinePlex Studio Messages The ta
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Table 1: CinePlex Studio Simple Mes
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Table 2: CinePlex Studio Critical M
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Plexon Inc Appendix C Lens Selectio
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C.2 Lens Selection For CinePlex Ver
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C.3 Experiment Design C.3.1 Field o
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The formula to calculate the optimu
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3 Three Arm Phenolic Knob (small) -
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• A local professional camera or
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a Place the sandwich plate on the o
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7 The tripod adaptor should already
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Port 1 Port 2 Port 1 Port 2 Port 3
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3 Using the Plexon camera mounting
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Plexon Inc Appendix D Plexon User I
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D.2 Screen Elements D.2.1 Menus The
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D.2.2 Toolbars Each toolbar has a d
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• Close Button - The Close button
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— Hidden Windows - Docked windows
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D.3 Standard Menu Items and Dialogs
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D.3.2 Run Menu The purpose of the R
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— Licensing - Clicking the Licens
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D.3.4 Messages Window The Messages
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D.4 Customization Although the menu
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• Category - This is a list of al
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• Buttons - The toolbar buttons a
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Plexon Inc Appendix E Cabling Guide
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E.2 Typical MAP/CPX Cabling Diagram
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E.3 Typical MAP/CPX System Cabling
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E.3.2 MAP/CPX System with Two Video
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E.4 MAP/CPX Cable Images Version 3.
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E.5.2.2 With CHUB Version 3.0 E-11
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E.5.3.2 Two Video Cameras Version 3
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* CinePlex, Plexon, and the Plexon
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