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CinePlex v3 Manual - Plexon Inc

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2<br />

that no pixels were in common with the previous frame. A value of 0 results<br />

when:<br />

• the frame is the first frame of the file,<br />

• the previous frame and the current frame are identical,<br />

• the tracking window switched from small in the previous frame to large in the<br />

current frame,<br />

• the tracking window switched from large in the previous frame to small in the<br />

current frame, or<br />

• the object in the previous frame and the object in the current frame are of 0<br />

size.<br />

<strong>CinePlex</strong> Studio writes the motion measure value to a digital VideoTracker<br />

(DVT) format file; for more information, see “Digital VideoTracker (DVT) Files”<br />

on page 156.<br />

The Motion Measure frame-to-frame pixel overlap technique provides information<br />

on the movement of the animal’s body over time. For example, a rotating<br />

animal’s centroid might not change, but the Motion Measure feature would indicate<br />

that the animal is not actually “frozen” because consecutive video frames are<br />

not identical.<br />

Note:<br />

The VTViewer motion measure chart does not move until the center of gravity<br />

is sent to the PLX file.<br />

To apply the Motion Measure option<br />

• On the Object Contour Tracker menu, click the Motion Measure checkbox.<br />

HINT<br />

Use Default Settings<br />

Use the default settings toolbar button to return all <strong>CinePlex</strong> Studio<br />

and camera settings to their default values. Set the default values<br />

prior to configuring a tracking mode.<br />

2.9.5 LED Mode Tracking<br />

This section includes the configuration procedure for configuring <strong>CinePlex</strong> Studio<br />

to track colored LEDs. The user may track up to three different colored LEDs.<br />

Each tracking mode operates separately. The user cannot combine Object Contour<br />

mode and LED mode. Use the Tracking Wizard (see “Object Contour Mode<br />

Tracking” on page 75) to provide an on-screen guide to the following procedure.<br />

82 <strong>CinePlex</strong>

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