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CinePlex v3 Manual - Plexon Inc

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name and click Export! The Export Complete! dialog box confirms the<br />

action.<br />

7 Use a text editor such as Windows Notepad to inspect the text file. Most other<br />

analyses programs can import the text format.<br />

4.6.5 Entering Tracker Coordinates<br />

Suppose that it is desirable to track the movement of some subtle feature on the<br />

video image. Something that <strong>CinePlex</strong> Studio cannot track automatically, like<br />

the position of the tip of a rat's tail.<br />

The procedural approach here is to define a new Coordinates marker, and use the<br />

Video window to enter—by mouse click—the coordinates that populate the new<br />

marker. Then, we can use the Interpolate Missing Occurrences tool to fill in the<br />

in-between frames.<br />

Entering tracking coordinates<br />

1 Define a new Coordinates marker (see “Defining a New Marker” on<br />

page 169). Label it “Tail Position”.<br />

2 In the Input window, after Left-Click, click Insert. <strong>CinePlex</strong> Editor is now<br />

“armed” to create a new occurrence of the “Tail Position” marker whenever<br />

clicking in the Video window.<br />

3 Decide how often coordinates need to be entered. The default case is to enter<br />

a Coordinates maker occurrence for each and every video frame. However,<br />

this means that the video must be advanced frame-by-frame, which can take<br />

a long time to do the tracking for an entire experimental run. If less accuracy is<br />

acceptable, it is possible to configure <strong>CinePlex</strong> Editor automatically to<br />

advance N video frames each time a coordinated is entered by clicking. This<br />

means that a “Tail Position” coordinate is entered only every Nth frame, but<br />

transversing the file occurs N times faster. From the Tools menu, select<br />

Options, and click the General tab in the Options dialog box. Type the value<br />

of N into the Number of Frames to Advance During Click-To-Add edit box.<br />

The default is N = 1, which enters a coordinate for every video frame.<br />

4 Click Play, then Pause, then Rewind to return to the beginning of the file.<br />

This ensures that <strong>CinePlex</strong> Editor is ready to take new coordinates.<br />

5 In the Video window, click at the position of the tip of the rat's tail. <strong>CinePlex</strong><br />

Editor enters a new marker occurrence, and advances N video frames.<br />

6 Repeat Step 5 until all the data needed has been gathered.<br />

7 When finished, in the Input window, at Left-Click, click Select to “disarm”<br />

<strong>CinePlex</strong> Editor.<br />

8 It is possible to generate automatically new “Tail Position” marker occurrences<br />

for the skipped frames by interpolating the coordinates between the ones<br />

entered. This is similar to “key-frame” animation techniques. <strong>CinePlex</strong> Editor<br />

interpolates the coordinates mathematically with no regard for the video<br />

images—the interpolated positions may or may not match the actual<br />

positions of the rat's tail on the skipped video frames. To perform this<br />

operation, from the Tools menu, select Interpolate Missing Occurrences;<br />

for more information, see “Interpolate Missing Occurrences” on<br />

Version 3.0 205

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