CinePlex v3 Manual - Plexon Inc

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2 2.1 Getting Started This section provides an overall procedure for using CinePlex Studio. Each step in the procedure is covered in detail in the subsequent sections. When embarking on a project, the user should have a detailed plan in place. The more detailed the plan, the more successful its execution will be. When using CinePlex Studio in an experiment, perform the following steps: • Designing the experiment - this step is concerned with animal selection, stimuli and rewards, physical design, environmental conditions, execution plan, and/or anything else pertinent to the overall design of the desired experiment. Detailed discussion of this step is beyond the scope of this manual. • Setting up the equipment - this step is concerned with setting up all of the equipment to be used in the experiment (CinePlex Studio workstation, Plexon NDAQ, computers, monitors, and any other peripheral equipment and their interconnection). • Performing an operational test - this step is concerned with checking out each piece of equipment, all connections, and all cables for proper operation. • Setting up CinePlex Studio - this step is concerned with positioning the camera so that the desired physical experiment area is within the field of view of the camera, deciding on the tracking mode to be used, and arranging the windows, setting the necessary properties of the selected tracking mode, determining file locations, synchronizing with other equipment, and any other considerations that may be pertinent. • Executing the experiment plan - this step is concerned with implementing the execution plan created in the Designing the experiment step. Detailed discussion of implementing the execution plan is beyond the scope of this manual. 16 CinePlex
2.2 Designing the Experiment The prime consideration from the CinePlex Studio point of view is whether CinePlex Studio will work properly with the experimental design. Some possible examples where CinePlex Studio may not work include: • The design of the experiment requires tracking of the entire body of a darkcolored animal in the dark. Contact Plexon for near infrared tracking options. • The ratio of the size of the trackable arena to the size of the animal is such that the animal’s image is too small or too large to be tracked. If assistance is needed in determining whether CinePlex Studio is applicable to the experimental design, please contact Plexon at 214-369-4957. Version 3.0 17
Page 1 and 2: Neurotechnology Research Systems Us
Page 3 and 4: CinePlex Video, Capture, Tracking,
Page 5 and 6: Plexon Inc Publication History iii
Page 7 and 8: Plexon Inc Contents v Publication H
Page 9 and 10: Plexon Inc CinePlex vii File Toolba
Page 11 and 12: Plexon Inc CinePlex ix Appendix B P
Page 13 and 14: Plexon Inc Chapter 1 CinePlex Syste
Page 15 and 16: — All-digital imaging with one or
Page 17 and 18: stamped with corresponding MAP time
Page 19 and 20: Editor features, see “Offline ext
Page 21 and 22: 1.3 CinePlex System Hardware and So
Page 23 and 24: The Quad MAP Controller (QMC) can c
Page 25 and 26: 1.4 Data Analysis After recording,
Page 27: Plexon Inc Chapter 2 Using CinePlex
Page 31 and 32: 2.4 Performing an Operational Test
Page 33 and 34: 4 Start Sort Client program and ver
Page 35 and 36: 7 Start CinePlex Studio program. Th
Page 37 and 38: will not be able to distinguish the
Page 39 and 40: 2.5.4.2.3 LEDs in Light with Reflec
Page 41 and 42: the object and the yellow rectangle
Page 43 and 44: 2.5.4.3.7 Multicolored Animal. The
Page 45 and 46: Selecting the Tracking Mode 1 Selec
Page 47 and 48: 3 Check the color to track (by clic
Page 49 and 50: that sizes, positions and velocitie
Page 51 and 52: d e Measure its longest dimension i
Page 53 and 54: In the above image note that the si
Page 55 and 56: d e Measure the feature’s horizon
Page 57 and 58: i If the current color in the box n
Page 59 and 60: n Go to the slider next to the Vert
Page 61 and 62: 6 Repeat these steps for each addit
Page 63 and 64: Defining Logical Events Logical eve
Page 65 and 66: 1 Click the Add new combination of
Page 67 and 68: 2.6 Executing the Experiment Plan T
Page 69 and 70: also use a variety of commercial so
Page 71 and 72: When the Start button is clicked, S
Page 73 and 74: Note: If Studio is armed on one day
Page 75 and 76: settings and use the file mode to d
Page 77 and 78: 2 To set or change the folder locat
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3 Open a second Windows Explorer an
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2.7 Global Configuration Pane The G
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2.8 Source Pane The Source Pane def
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The Arena Definition is also used t
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• In Reflective Colors mode, pixe
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Configuring CinePlex Studio to trac
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emove progressively larger features
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The illustration below is taken aft
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Configuring CinePlex Studio to trac
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2 In the Video window a square area
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If Pure Colors is not selected, the
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Increase the threshold to reduce th
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2.10 Operating CinePlex Studio with
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To verify CinePlex Studio data acqu
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3 In the Control View, click Connec
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2.10.1.1 Control View VTViewer disp
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2.10.1.3 VTViewer Options The Optio
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2.11 CinePlex Studio Offline Mode 2
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• Sequences Pane(s) (if behavior
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2.13 CinePlex Studio Test Mode Test
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2.14 Operation with the Quad MAP Co
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6 Connect a push button switch (09-
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Version 3.0 111
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15 On each CPX, launch and arm CPX.
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2.15 Using the Extractor This chapt
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illustrates. Note that the Marker 4
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Plexon Inc Chapter 3 CinePlex Studi
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3.2.1 Static Configuration Pane The
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3.2.1.2.1 Camera Group. The Camera
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• Brightness - this setting speci
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3.2.1.3 Tracking Tabs One of three
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3.2.1.4.1 Object Contour Tracker Gr
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• Object Transparency - This sett
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• Cent. Grav. Size - This setting
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• Mrk1Threshold, Mrk2Threshold, M
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3.2.2 Dynamic Configuration Pane Th
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• Subtract shapes - - this button
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• Add zone to selected sequence -
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• Delete selected tracking event
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3.2.2.4.1 Toolbar. The toolbar cont
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Note: The effects of the Image Qual
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— Name - this value depends on wh
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3.3 Menus The CinePlex Studio File
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3.3.3 DVR Menu 3.3.3.1 Arm Clicking
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3.4 Toolbars The File, DVR and Trac
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ow,time,x1,y1,x2,y2,x3,y3,x4,y4,x5,
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3.6.3 Source Settings The Source se
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Plexon Inc Chapter 4 Using CinePlex
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4.1.3 Changing and Saving Project F
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4.2.2 Setting Up a New Project This
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4.2.3 Understanding the CinePlex Ed
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4.2.4 Defining a New Marker This pr
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Markers window in the Name column a
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3 In the Format area, accept the de
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4.4 Customizing the User Interface
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In the Input window, select an inpu
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matically advances the video by a p
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4.5.3 Editing Markers and Marker Oc
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Selected Marker Occurrence, or clic
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3 Select entries in the Marker 1 an
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is defined and selected. A more sop
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corner of the image. If the video r
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Finding marker occurrences with a b
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2 Choose a marker from the Marker l
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Interpolating missing occurrences 1
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lowing illustration idx indicates t
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6 The user may set the columns for
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4.6 CinePlex Editor How-Tos This se
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However, do not expand the range so
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name and click Export! The Export C
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7 Repeat Step 6 until all the coord
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Plexon Inc Chapter 5 CinePlex Edito
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5.2 Main Window This section descri
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Show Complete Tail feature is usefu
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The color drawn at point p in the t
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— By pressing the PAGE UP or PAGE
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• Automatically Adjust Label Area
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Note: — Type - In the Type area,
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ox and select a different key. When
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Note: By default, if the Show check
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— Data Type: - This item contains
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5.2.8.1 Function The Messages windo
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• Add, Edit, and Delete Marker Oc
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• Reset Automatic Range Adjustmen
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5.3 Menus Menus provide access to C
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— Video Data Input File (AVI) - T
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After verifying that the correct fi
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• Which Markers - The default val
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• File - Type an export file name
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5.3.2 Edit Menu The following illus
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Note: The Edit Marker dialog box ma
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• Marker - This box shows the mar
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5.3.4.7 Step Forward This button ad
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5.3.6 Find Menu The following illus
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• Generate Selected - This button
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• Generate All - This button gene
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— Zone Interval • Operation to
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5.3.7.5 Snap-To Marker Occurrences
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— max - the largest time differen
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5.3.7.8.1 General Tab. • Initial
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5.3.7.8.3 Latency Correction Tab. F
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5.3.7.8.5 Audio Tab. • Available
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— Background - This is the backgr
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5.4 Toolbars CinePlex Editor includ
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5.5 How CinePlex Editor Creates Ini
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then load a neural data file that c
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Marker Type Abbr Information Stored
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From the Marker Occurrences window
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Note that applying this correction
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Snap-To Marker Occurrences tool des
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5.10 Analyzing CinePlex Studio data
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Plexon Inc Appendix A Installation
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Procedure for MAP/CPX Computer Hard
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A.2 Installation - Software This se
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3 The End User License Agreement wi
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7 The Select Program Manager Group
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11 The following window appears twi
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14 Click Next on the window below.
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18 The CinePlex installation has no
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3 The End User License Agreement wi
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7 The Select Program Manager Group
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11 Next, the Sentinel Key Drivers a
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15 Click Finish on the window below
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2 The Welcome window appears. Press
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6 The Select Components window appe
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10 Nothing else will be installed,
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3 After step 6 continue the procedu
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Note: to remote upgrades. If a remo
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Plexon Inc Appendix B Problem Resol
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toggle switch above these connector
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the CinePlex software will be requi
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1 Remove the TIM card and check tha
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1 Stop running all unnecessary appl
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B.3 CinePlex Studio Messages The ta
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Table 1: CinePlex Studio Simple Mes
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Table 2: CinePlex Studio Critical M
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Plexon Inc Appendix C Lens Selectio
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C.2 Lens Selection For CinePlex Ver
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C.3 Experiment Design C.3.1 Field o
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The formula to calculate the optimu
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3 Three Arm Phenolic Knob (small) -
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• A local professional camera or
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a Place the sandwich plate on the o
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7 The tripod adaptor should already
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Port 1 Port 2 Port 1 Port 2 Port 3
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3 Using the Plexon camera mounting
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Plexon Inc Appendix D Plexon User I
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D.2 Screen Elements D.2.1 Menus The
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D.2.2 Toolbars Each toolbar has a d
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• Close Button - The Close button
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— Hidden Windows - Docked windows
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D.3 Standard Menu Items and Dialogs
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D.3.2 Run Menu The purpose of the R
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— Licensing - Clicking the Licens
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D.3.4 Messages Window The Messages
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D.4 Customization Although the menu
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• Category - This is a list of al
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• Buttons - The toolbar buttons a
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Plexon Inc Appendix E Cabling Guide
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E.2 Typical MAP/CPX Cabling Diagram
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E.3 Typical MAP/CPX System Cabling
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E.3.2 MAP/CPX System with Two Video
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E.4 MAP/CPX Cable Images Version 3.
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E.5.2.2 With CHUB Version 3.0 E-11
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E.5.3.2 Two Video Cameras Version 3
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* CinePlex, Plexon, and the Plexon
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CinePlex v3 Manual - Plexon Inc

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