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CinePlex v3 Manual - Plexon Inc

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4<br />

page 262. From the Marker list, select Tail Position. Because we want<br />

coordinates for every frame, in the Timestamp Determination area, click<br />

Use Frame Marker Times. Click OK to close the dialog box and enter the<br />

tracking coordinates.<br />

4.6.6 Repairing Tracking Coordinates<br />

Suppose that <strong>CinePlex</strong> Studio is used to track an LED mounted to the headstage<br />

on a rat. Occasionally, the cable from the headstage can get between the LED and<br />

the camera, so the position data for the LED is missing in some frames. Or, suppose<br />

something caused the tracker to produce bad coordinates for a few frames,<br />

possibly caused by some light leaking into the experimental setup that confused<br />

the tracking algorithms.<br />

In either case, the procedural approach is to put <strong>CinePlex</strong> Editor into a state<br />

where the video is advanced frame-by-frame and enter new or replacement<br />

tracker coordinates by clicking in the Video window at the desired location.<br />

Repairing tracking coordinates<br />

1 In the Markers window, select the Coordinates marker to repair. Click Show.<br />

To minimize confusion during the procedure, clear Show for other Coordinate<br />

markers (if present).<br />

2 For the following procedure to work, the Coordinates markers must be aligned<br />

to the Frame marker times by using the Snap-to Marker Occurrences dialog<br />

box, for more information, see “Snap-To Marker Occurrences” on<br />

page 193. Follow the procedure outlined in that section to align the<br />

Coordinate markers to the Frame markers.<br />

3 Locate a section of time that has bad or missing values for the selected<br />

Coordinate marker that needs to be repaired. Pause the playback at the first<br />

frame with bad or missing coordinates.<br />

4 From the Tools menu, click Options, and in the Options dialog box click the<br />

General tab. Make sure that the the selection in the Number of Frames to<br />

Advance During Click-To-Add box is 1 (the default). Also, from the Animate<br />

menu, select Lock To Video Frames.<br />

5 In the Input window, at Left-Click, click Insert. This “arms” <strong>CinePlex</strong> Editor<br />

to enter new (or replace existing) marker occurrences when clicking the left<br />

mouse button in the Video window.<br />

6 Position the mouse cursor where the new (or replacement) coordinate needs<br />

to be and click.<br />

The position is entered (or updated) and the video automatically advances by<br />

one frame.<br />

206 <strong>CinePlex</strong>

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