LabChip GX/GXII User Manual

Software Reference 144Peak Table View (Continued)CommentDisplays any comments associated with the well, either from theSample Name File selected in the Advanced Tab on the Start RunWindow or from the Sample Name Editor Window.Signal NoiseThe statistical standard deviation of 1 second of data just before thePeak Find Start Time.BaselineThe center average height of the peak baseline, measured in RFUs.FWHMThe width of the peak (Full Width at Half Maximum), measured inseconds.Molarity (nmol/l)(DNA Assays only) Displays the molarity of the peak.Corr. Area (Glycan Assays) and Time Corr. Area (Protein Assays)Displays the mobility corrected area. The area of a peak shouldrepresent the total area of the compound responsible for thefluorescence. In the Caliper instrumentation, the fluorescence of thecompound moving past the detector is sampled at constant timeintervals unlike the conventional slab gel where the compound isspread out spatially and imaged at a single time. To convert from apeak area integrated in the time dimension to one integrated in thespace dimension, we use the fact that x is proportional to µt so ∆xα µ∆t and the mobility µ is proportional to 1/T where T is themigration time of the peak. So the Time Corr Area = 100 * Area/T.The 100 is an arbitrary constant designed to keep the correctedarea in a range similar to the original area computed from the timeseries data.RNA Fragment(RNA Assays only) The name of the fragment based on its sizefalling within a certain range set in the assay definition. (Thesedefinitions are visible only to assay developers).Fragment Area(RNA Assays only) The sum of the trapezoids between thefragment start and end times. The trapezoid base is a line drawnacross the fragment base from the start point to the end point.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.