LabChip GX/GXII User Manual

Glossary of Terms 259Peak BaselineA local peak baseline is calculated for each peak. For isolatedpeaks, the local peak baseline is a straight line connecting the startpoint with the end point. For peaks that are very close together, anaverage baseline is used when the value between the peaks doesnot drop to the actual baseline. The peak baseline for each peak inFigure 118 is shown in a different color.Peak HeightFigure 118. Peak BaselinesThe baseline algorithm starts at the earliest peak and checkswhether the end point is within a certain distance from the start ofthe next peak. When a cluster of peaks is detected, the baseline isthe line joining the first peak's start to the last peak's end. The startand end points of adjacent peaks in the cluster are averaged to thesame point so that no gaps exist between peaks.The peak baseline Start Point and End Point can be moved in theOverlay Electropherograms Tab if Show Peak Baselines isselected in the Graph View Properties.The value at the apex of the peak minus the local baseline startvalue.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.