LabChip GX/GXII User Manual

Operation 27Monitoring the RunThe following occurs after a run is started in the LabChip GXsoftware:1 The priming and warming steps are performed.• The priming step fills the channels of the chip with reagent.(Only performed if the chip holder has been opened sincethe last run.)• The warming step allows the heater plate located in the chipholder to regulate chip temperature to 30°C.• Data collection begins after the warming and priming stepsare completed.2 After the run begins, the Start button on the LabChip GX mainwindow changes to Stop.3 The Active Data Tab displays the electropherogram of the wellcurrently being read. Data is saved to a file with the nameshown above the plate diagram after each well is completed.4 To view the results for individual wells as data is acquired orafter the run is finished, click a well in the Plate View or PlateList, a sample name in the Well Table View, or a lane in the GelView. Data from the selected well displays in the OverlayElectropherograms Tab and the Peak Table View.For more information on data analysis, see “Data Analysis” onpage 35.5 To stop the run before it is complete, see Stopping a Run.6 When the assay is complete, Run Successfully Completeddisplays in the Status line.7 If desired, remove the plate and/or remove the chip.To save the analyzed data:1 Select File Save Workspace. The Save Workspace AsWindow opens with the default workspace name as the name ofthe data file.2 If desired, change the location and/or the name of theworkspace file and click the Save button. (Workspace files havea .gxw file extension.)To view or re-analyze the data, see Data Analysis.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.