LabChip GX/GXII User Manual

Data Analysis 39How the Software Analyzes Protein Data(Continued)4 A sizing ladder (see Figure 8), which is a mixture of proteinfragments of different known sizes, is run from the ladder vialbefore and after every 12 samples. The sizes of the individualproteins (in kDa) are preset in the assay and cannot bechanged.Figure 8. Ladder Graph - Protein5 The ladder is analyzed (see “Ladder Analysis” on page 257) anda standard curve of migration time versus mobility is plottedfrom the ladder by interpolation between the individual proteinsize/migration points. The standard curve derived from the dataof the ladder well should resemble the Figure 9.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.