LabChip GX/GXII User Manual

Data Analysis 49How the Software Analyzes Glycan Data(Continued)7 The standard curve and the markers are used to calculateglycan sizes in each well from the migration times measured.8 The area under each peak is calculated.9 All the sample peak areas are added together and an area ratiois calculated for the %Area for each peak.NOTES• In Glycan assays, the lower marker does not precede all ladderpeaks but rather appears between the 6th and 7th ladder peak.The concentration of the lower marker has been madesufficiently large to avoid confusion with ladder peaks. Sincepeaks before the lower marker are ignored, the ladder peaksused for the sizing ruler range from 7 to 13 CGU.• The software allows you to define the lower marker. Changingthe selection of the lower marker will lead to quantitativechanges in the calibration, and therefore in the entire dataevaluation.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.