LabChip GX/GXII User Manual

Software Reference 155Alignment TabThe Alignment tab on the Assay Analysis Window is used to assignalternate ladders to a specified group of wells in the event of afaulty ladder result. The default assignment for full rows uses theladder adjacent to the row where the sample is located. If thisladder cannot be analyzed, the software automatically looks foranother nearby ladder to perform the analysis. The actual ladderused for the analysis is shown in the Well Peak Find Settings panelof the Peak Find Tab. It can be changed by selecting the “AlignWell Groups to Specified Ladder” option and then selecting adifferent ladder in the “To Ladder” column corresponding to the rowto be changed.For Protein Assays, which do not use an upper marker, alignment isnormally performed with ladders on each end of the row tocompensate for drift between start and end of each row. Thisfeature can be disabled by selecting “Align Well Groups toPrevious Ladder” or “Align Well Groups to Specified Ladder”instead of “Align Well Groups to Bracketed Ladder”.Figure 58. Assay Analysis Window - Alignment TabThe Alignment tab contains the following settings:• Align Well Groups to corresponding Ladders - If selected,each row is aligned to the ladder at the beginning of the row.• Align Well Groups to Bracketing Ladders - (Protein assaysonly.) If selected, each row is aligned with the ladder at thebeginning of the row and a ladder at the end of the row. (Proteinassays are only supported on LabChip GX II instruments.)P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.