LabChip GX/GXII User Manual

Software Reference 171Advanced TabUse the Advanced tab on the Assay Analysis Window to view thequantification marker settings or change the Peak Size Calibrationpoint.Figure 65. Assay Analysis Window - Advanced TabQuantificationDetermines whether the upper or lower marker is used forquantitation. Protein and RNA assays use the lower marker. DNAassays use the upper marker. DNA and RNA Quantificationselections are set by the assay properties and cannot be changed.For Protein assays, selecting Sample Peak uses the peak size andconcentration of the User Standard instead of the default laddersettings. Not available for Glycan assays. (Protein and Glycanassays are only supported on LabChip GX II instruments.)Peak Size Calibration PointSpecifies which part of the peak is used as the size calibrationpoint.• Leading Edge - If selected, uses the start of the peak. (May bebetter for data where sample peaks are broader than ladderpeaks.)• Center - If selected, uses the top of the peak. (May be better fordata where leading edge is not well-defined.)Concentration Size Correction (Protein assays only)Protein concentration is size-corrected to account for differences inthe amount of protein injected into the separation channel based onits size. The correction is obtained using the formula:Conc(Size Corrected) = Conc(Time Corrected Area) * Amplitude *(Size)^PowerP/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.