LabChip GX/GXII User Manual

Data Analysis 45How the Software Analyzes RNA Data(Continued)8 The Total RNA present is computed by finding the area underthe electropherogram trace. The baseline for this integration is astraight line starting at the end of the lower marker and endingat the baseline end time. The height of the baseline endpoints iscomputed from an average of a five second window around thebaseline Start Time and End Time (shown on the Peak FindTab). View the baseline by selecting Show Peak Baselines inthe Graph View Properties. Adjust the Start Time and End Timeby dragging the left (Start) and right (End) vertical dashed linesto areas that more properly reflect the signal baseline. (Rightclickin the graph, select Set Scale, and change the X axisMinimum and Maximum values if the start and end times are notshown in the graph.)Figure 14. Peak Baseline - RNA9 The Total RNA concentration in the sample is computed fromthe ratio of the RNA area in the sample to the RNA area in theladder multiplied by the ladder concentration specified in theassay.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.