LabChip GX/GXII User Manual

Glossary of Terms 265SmearSpecifies a size range in which to detect the region of thefluorescence signal instead of specifying a specific size for a peak.Use a smear to detect a broad mix of molecules of similar butdistinct sizes in a sample. The concentration or fractional presencewithin a specified range of molecule sizes is measured. Specify theStart Size and End Size of a smear in the Assay Analysis Windowon the Smear Analysis Tab.The Smear Properties can be viewed in table form in the Well TableView. To view the Smear Properties in the table, add the desiredproperty to the Well Table View. The properties extracted from thesmear match those of a peak, having extends, area, concentration,etc. % Purity is the ratio of smear concentration to total samplepeak concentration, exclusive of markers, system peaks, andexcluded peaks. Excluded peaks are not excluded from analysiscalculations such as concentration or area within the smear region,so the limits must avoid excluded peaks. An additional Peak Countproperty is available for smears. Peak Count is the number of nonexcludedsample peaks in the region, where the center of the peakis inside the smear limits.In the Graph View, the smear regions display with the integrationextends drawn at the base of the smear in the chosen color and thetrace highlighted with the smear color. Override the smear base bydragging with the mouse in the Graph view. Only the verticalposition of the base corners can be adjusted in the graph, since thesize limits are specified and should not be altered.In the Gel View, the smear region is shown on the gel as a colorizedregion. The color is a transparent version of the color selected forthe region, so the fluorescence intensity still shows through.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.