LabChip GX/GXII User Manual

Data Analysis 63Changing the Peak Find ParametersAfter data filtering, the peak find algorithm locates the peaks andcalculates the local peak baselines. The algorithm begins by findingall the peaks above the noise threshold to determine the baseline,after which any peaks below the noise threshold are rejected. Alocal baseline is calculated for each peak to allow for baseline drift.The following Peak Find parameters can be changed:• Min Peak Height• Min Peak Width• Slope Threshold• Inflection Threshold• Start Time• End Time• Filter Width• Baseline PlateauTo change the Peak Find parameters for all wells:1 Select Analysis Analysis Settings to open the AssayAnalysis Window, and then click the Peak Find Tab.2 Change the parameters as necessary at the top of the window.3 Click the OK button to save the setting, reanalyze the data, andclose the window.Click the Apply button to apply the changes and reanalyze thedata, but keep the Assay Analysis window open.Click the Apply Global button the apply the settings to all platesin the workspace and reanalyze the data, but keep the AssayAnalysis window open.To change peak find settings for individual wells:1 Select Analysis Analysis Settings to open the AssayAnalysis Window, and then click the Peak Find Tab.2 In the Well drop-down list, select the well number that you wantto change the settings for.3 Change the settings at the bottom of the window under WellPeak Find Settings to change the settings for the selected well.4 Click the OK button to save the setting, reanalyze the data, andclose the window.Click the Apply button to apply the changes and reanalyze thedata, but keep the Assay Analysis window open.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.