LabChip GX/GXII User Manual

Data Analysis 37How the Software Analyzes DNA Data(Continued)5 The ladder is analyzed (see “Ladder Analysis” on page 257) anda standard curve of migration time versus DNA size is plottedfrom the DNA ladder by interpolation between the individualDNA fragment size/migration points. The standard curve derivedfrom the data of the ladder well should resemble Figure 6.Figure 6. Standard Curve Window - DNA6 Two DNA fragments are run with each of the samples,bracketing the DNA sizing range. The Lower Marker and UpperMarker are internal standards used to align the ladder data withdata from the sample wells. Figure 7 shows an example ofassigned marker peaks in a sample well.Figure 7. Markers - DNAP/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.