LabChip GX/GXII User Manual

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Glossary of Terms 254Inflection Threshold ExamplePeaks that are very close together will be identified as a single peakif the peaks do not have a clear valley between them. The Inflectionthreshold property allows splitting of peaks evidenced by a region oflower slope. The figures below illustrate the operation of theInflection threshold.The green line in the figures below shows the signal slope. Theshoulder peaks have a slope minuma (inflection point) but the slopedoesn't change sign so they are not split into multiple peaks via theslope threshold.Figure 114. Slope Threshold 0The inflection threshold defines the value that the slope minimummust be below to trigger a splitting of the peak. As the threshold isincreased, more peak splitting occurs. The figures below show thesame data with the slope threshold set to 80 and 200.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.
Glossary of Terms 255Figure 115. Slope Threshold 80Figure 116. Slope Threshold 200P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.
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LabChipGX/GX IIUser ManualCopyright
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Preface 3For support in Europe cont
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Preface 5Table 1. Important Symbols
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Instrument Safety 7Required Trainin
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Instrument Safety 9Laser SafetyWARN
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Instrument Safety 11InternationalAl
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Table of Contents 13Viewing the EFs
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Table of Contents 15Login Window...
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Introduction 17IntroductionThis man
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Introduction 19Principles of Operat
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Operation 21OperationThis section i
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Operation 23Running an AssayTo star
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Operation 25Running an Assay (Conti
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Operation 27Monitoring the RunThe f
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Operation 29Continuing a Stopped Ru
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Operation 31Saving Data Files (Cont
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Operation 33Adding a New Plate (Con
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Data Analysis 35Data AnalysisAfter
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Data Analysis 37How the Software An
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Data Analysis 51Opening a Data File
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Data Analysis 53Selecting the Wells
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Data Analysis 55To import the sampl
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Data Analysis 57Using Expected Frag
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Data Analysis 59Forcing Expected Pe
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Data Analysis 61Viewing the EFs/EPs
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Data Analysis 63Changing the Peak F
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Data Analysis 65Merging Two PeaksIf
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Data Analysis 67Using the Default L
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Data Analysis 69Upper and Lower Mar
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Data Analysis 71Lower Marker Peaks
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Data Analysis 73Viewing GelsTo comp
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Data Analysis 75Adjust Pane WidthsT
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Data Analysis 77Viewing Graphs in t
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Data Analysis 79Viewing Multiple Pr
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Data Analysis 81Reanalyzing a Data
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Data Analysis 83ElectropherogramCho
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Data Analysis 85Peak TableChoosing
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Data Analysis 87Raw DataASCII text
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Data Analysis 89Exporting Data Manu
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Software Security 91• “Backing
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Software Security 93Managing User A
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Software Security 95Changing User I
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Software Security 97Changing Access
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Software Security 99Setting Policie
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Software Security 101Electronic Sig
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Software Security 103Audit TrailLab
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Software Security 105Exporting the
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Software Security 107Creating New D
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Software Security 109Remote CDR Ser
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Software Security 111Backing Up Dat
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Software Reference 113Software Refe
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Software Reference 115Menu BarThe m
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Software Reference 117Collection Me
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Software Reference 119Security Menu
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Software Reference 121Chip Status a
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Software Reference 123Error Message
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Software Reference 125Rename Plate
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Software Reference 127Collection Pa
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Software Reference 129Graph View (C
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Software Reference 131Active Data T
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Software Reference 133Electropherog
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Software Reference 135OptionText Or
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Software Reference 137Gel View (Con
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Software Reference 139Gel View Prop
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Software Reference 141Peak Table Vi
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Software Reference 147Filter ViewTh
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Software Reference 149Filter View (
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Software Reference 151Add New Expec
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Software Reference 153Assay Analysi
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Software Reference 155Alignment Tab
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Software Reference 157Analysis Tab
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Software Reference 159Peak Find Tab
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Software Reference 161Table 2. Well
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Software Reference 163Expected Frag
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Software Reference 165Excluded Peak
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Software Reference 167Smear Analysi
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Software Reference 169Titer Tab (Co
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Software Reference 171Advanced TabU
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Software Reference 173Audit Trail W
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Software Reference 175Audit Trail M
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Software Reference 177DeleteButtonH
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Software Reference 179CDR Utility W
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Software Reference 181Change Passwo
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Software Reference 183Event Viewer
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Software Reference 185Export Window
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Software Reference 187Installation
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Software Reference 189Login WindowU
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Software Reference 191Perform Signa
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Software Reference 193Print WindowU
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Software Reference 195Print Validat
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Software Reference 197Run File Edit
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Software Reference 199Sample Name E
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Software Reference 201Save Workspac
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Page 207 and 208: Software Reference 207Output Tab (C
Page 209 and 210: Software Reference 209Advanced Tab
Page 211 and 212: Software Reference 211System Diagno
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Page 215 and 216: Software Reference 215Edit UsersUse
Page 217 and 218: Software Reference 217De/Activate U
Page 219 and 220: Software Reference 219Show/Hide in
Page 221 and 222: LabChip GX Instrument Description 2
Page 231 and 232: Maintenance and Service 231Maintena
Page 233 and 234: Troubleshooting and Diagnostics 233
Page 247 and 248: Glossary of Terms 247Glossary of Te
Page 249 and 250: Glossary of Terms 249CLAThe file ex
Page 251 and 252: Glossary of Terms 251Data Filtering
Page 253: Glossary of Terms 253GXD FilesThe f
Page 257 and 258: Glossary of Terms 257Ladder Analysi
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Page 261 and 262: Glossary of Terms 261Polynomial Ord
Page 263 and 264: Glossary of Terms 263For the purpos
Page 265 and 266: Glossary of Terms 265SmearSpecifies
Page 267 and 268: Glossary of Terms 267Figure 120. Pr
Page 269 and 270: Glossary of Terms 269WorkspaceZero
Page 271 and 272: Caliper Life Sciences, Inc. Product
Page 273 and 274: Caliper Life Sciences, Inc. Softwar
Page 275 and 276: Index 275IndexNumerics21 CFR Part 1
Page 277 and 278: Index 277showing. . . . . . . . . .
Page 279 and 280: Index 279clearing default ladder .
Page 281 and 282: Index 281Remote CDR Server . . . .
Page 283 and 284: Index 283Waste wells . . . . . . .
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