LabChip GX/GXII User Manual

Glossary of Terms 248BaselineA baseline is established just after the start time setting. (For DNAand RNA assays, you can change the Baseline Start time on theAnalysis Tab.) After the overall baseline is established, a localbaseline is calculated for each peak to compensate for baselinedrift.For isolated peaks, the local peak baseline is a straight lineconnecting the start point of the peak with the end point. For peaksthat are very close together, an average baseline is used when thevalue between the peaks does not drop to the actual baseline.To select the desired baseline algorithm for all of the samples in theplate, choose the desired option on the Peak Find Tab: No baseline,Zero Baseline, or Baseline subtraction.Figure 113 shows baselines established for DNA assay peaks(based on the settings in the Peak Find Tab). DNA peaks aredetermined on a peak-by-peak basis (the overall baseline isshown).Figure 113. BaselinesBubbleIf the tip of a pipette is not positioned below the liquid level in thewell, bubbles can result. If a large bubble forms at the bottom of awell, remove the sample from the well, pipette the sample back intothe well, and continue with the loading procedure.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.