LabChip GX/GXII User Manual

Software Reference 160Table 1. Plate Peak Find SettingsPlateSettingsExcludeSystemPeaksBaselineAlgorithmHigh SizeFilteringFunction(Protein and Glycan assays only) If selected, excludes the systempeak (peak due to buffer constituent) from any changes you make tothe peak find settings (the most recent custom ladder settings areused instead).• None - No correction.• Zero Baseline - Offsets all graphs to zero baseline but does notaffect analysis.• Baseline Subtraction - A dynamic subtraction of the baseline thatcorrects for drifting Baseline. This algorithm creates a smooth linefit to the baseline data points and subtracts this smooth fit from thedata. The threshold for the baseline correction allows you todetermine how much the baseline fit tries to follow changes in thedata. Lowering the Threshold below the default value of 20 allowsthe baseline fit to ignore regions that are slow changes of realsignal peaks and not baseline drift.If selected, a second, larger filter is applied to the data after thespecified time. This is useful for assays where the peaks are narrowat the start of the assay and become broad and noisy for larger sizesof DNA, protein or Glycan. If not selected, the same size filter is usedfor all data.• Filter Width (sec) - The width of the second filter to be applied afterthe specified transition time. Filter Width must be greater than theSample Well Filter width and less than or equal to 3.0 seconds.• Transition Time (sec) - The time at which to transition to the largerfilter size. Transition time must be greater than the Baseline Timeon the Analysis tab and less than the Well End time.Table 2. Well Peak Find SettingsWellSettingsWellLadderMinimumPeak HeightFunctionDisplays the well being edited. Select any well or ladder on the platebelonging to the current collection.Read only. Displays the ladder used for aligning this well. UseAlignment tab to edit. New value displays here after clicking Apply.Specifies an override to the Peak Find Settings for Minimum PeakHeight for the entire plate. Specify a valid numeric value to override,or clear the field to reset to the Global setting.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.