LabChip GX/GXII User Manual

Glossary of Terms 254Inflection Threshold ExamplePeaks that are very close together will be identified as a single peakif the peaks do not have a clear valley between them. The Inflectionthreshold property allows splitting of peaks evidenced by a region oflower slope. The figures below illustrate the operation of theInflection threshold.The green line in the figures below shows the signal slope. Theshoulder peaks have a slope minuma (inflection point) but the slopedoesn't change sign so they are not split into multiple peaks via theslope threshold.Figure 114. Slope Threshold 0The inflection threshold defines the value that the slope minimummust be below to trigger a splitting of the peak. As the threshold isincreased, more peak splitting occurs. The figures below show thesame data with the slope threshold set to 80 and 200.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.