LabChip GX/GXII User Manual

Data Analysis 57Using Expected Fragments/ Expected Proteins/Expected GlycansYou can track expected DNA fragments (EFs), proteins (EPs), orGlycans (EGs) for the samples in a DNA, Protein, or Glycan assay,respectively. (Protein and Glycan assays are only supported onLabChip GX II instruments.) You enter the EFs, EPs, and EGs in theAssay Analysis Window.Entering EFs, EPs, or EGs in the Assay Analysis Window1 Select Analysis Analysis Settings on the Menu Bar. TheAssay Analysis Window opens.2 Click on the Expected Fragments/Proteins/Glycans Tab.3 Click in the bottom (empty) row in the table.4 In the column labeled Size, enter the size of the expectedfragment/protein/glycan in bp, kDa, or CGU, respectively.5 In the Window (%) column, if desired, change the tolerancevalue to allow for variations in the expectedfragment/protein/glycan size. This value is specified as apercent of the expected size for that fragment/protein/glycan.The default is 10% for DNA and protein, and 2.5% for Glycan.Note: If there are multiple peaks in the tolerance range, thelargest peak is labeled as the expected peak, even if it is not theexact size specified. To change the peak identified as anexpected peak, see “Forcing Expected Peaks” on page 59.6 A default color is automatically assigned. To change the color,click on the color block in the Color column and select thedesired color in the Color window.7 If desired, change the name shown in the Name column.8 If desired, change the Property Displayed in the Well Table.This setting specifies the property that will be displayed in theExpected Peak column for each expected peak listed in the WellTable View.9 For Protein assays only, to align the data to one or more specificpeaks, select the For Aligning check box. The data is realignedso the selected peaks match their aligned size. Note: Incorrectalignment settings can cause analysis errors.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.