LabChip GX/GXII User Manual

Glossary of Terms 268Start PointStart TimeTiterTool TipUpper MarkerThe peak find algorithm analyzes the data from time zero lookingfor a slope greater than the Slope Threshold. The point where theslope is greater than the slope threshold specified in the Peak FindTab is marked as the start point of a peak.This setting determines the time after the start of a run when peakswill be detected. Any peaks appearing before this time are ignored.Allows the use of a standard with a known concentration to be usedas a calibration standard instead of using a ladder. The well locationand known concentrations of the standard are specified in theAssay Analysis Window on the Titer Tab.The Titer Standard Curve used for calibration is normalized againstthe lower marker (protein and RNA) or upper marker (DNA) toadjust for slight variations in each well. A normalization standardcan be added to each well to be used only for normalization.(Normalization standards must be the same concentration in eachwell.) You choose the normalization Standard in the Assay AnalysisWindow on the Advanced Tab. Titers can also be imported fromanother plate if the selected Normalization Standard is the same. IfSample Peak is selected, the Sample Peaks must be the samesize.The Concentration to Favor in Fit adjustment on the Titer tabenables you to choose whether to weight either the higher or lowerconcentrations more heavily when calculating the Titer StandardCurve.A small box containing text that describes the item indicated by themouse pointer. To view a Tool Tip, position the cursor over an itemon the window. Leave the cursor stationary for a moment and aTool Tip (if one exists for that item) displays.An internal standard that is added to a DNA sample in a well toassist in determining size and concentration of the sample. Themarker is the same as the last peak in the DNA ladder.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.