LabChip GX/GXII User Manual

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LabChip GX Instrument Description 224OpticsThe LabChip GX optics provide fluorescence detection (redexcitation and red emission) for DNA, RNA, Protein and Glycanchips and assays. (Protein and Glycan assays are only supportedon LabChip GX II instruments.)Red LaserThe LabChip GX instrument includes a high intensity, long-life reddiode laser to excite fluorescence on microfluidic chips. It isexpected to have a lifetime of tens of thousands of operating hours.Focusing and AlignmentThe LabChip GX instruments use back-reflected red diode laserlight for automatic chip focusing.Photodiode DetectorsThe LabChip GX and LabChip GX II use two silicon photodiodedetectors for fluorescence detection and autofocus. Fluorescencedata can be acquired and stored at rates from 20 to 120 Hz.Optical TrainThe fluorescence excitation, fluorescence detection, and autofocusoptical trains contain several lenses and high efficiency interferencefilters. The optical trains are factory aligned and do not requireadjustment.Chip Pressure SystemBarcode ReaderThe pressure pump applies pressure or vacuum to the wells on thechip to move liquid through the chip. The Pressure Pump is locatedinside the LabChip GX instrument. O-Rings on the Chip Cartridgeseal the chip wells and maintain the pressure.The LabChip GX instrument is equipped with an internal BarcodeReader. The Barcode Reader reads the customer-applied barcodeon the short (portrait) edge of the microplate.Proper selection and placement of barcode labels is critical forsuccessful reading. See “Placing the Barcode on the Plate” onpage 34 for specifications.The barcode reader is internal to the system and cannot be viewedfrom the outside.P/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.
LabChip GX Instrument Description 225DNA, RNA, and Protein ChipsThe figures below show the format and parts of the microfluidicchips used in the LabChip GX instruments to perform DNA, Protein,RNA, and Glycan sizing and quantitation. (Protein and Glycanassays are only supported on LabChip GX II instruments.)Figure 106. Top of ChipFigure 107. Bottom of ChipP/N 127170 Rev. 00 LabChip GX User Manual Caliper Life Sciences, Inc.
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LabChipGX/GX IIUser ManualCopyright
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Preface 3For support in Europe cont
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Preface 5Table 1. Important Symbols
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Instrument Safety 7Required Trainin
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Instrument Safety 9Laser SafetyWARN
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Instrument Safety 11InternationalAl
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Table of Contents 13Viewing the EFs
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Table of Contents 15Login Window...
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Introduction 17IntroductionThis man
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Introduction 19Principles of Operat
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Operation 21OperationThis section i
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Operation 23Running an AssayTo star
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Operation 25Running an Assay (Conti
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Operation 27Monitoring the RunThe f
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Operation 29Continuing a Stopped Ru
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Operation 31Saving Data Files (Cont
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Operation 33Adding a New Plate (Con
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Data Analysis 35Data AnalysisAfter
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Data Analysis 37How the Software An
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Data Analysis 51Opening a Data File
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Data Analysis 53Selecting the Wells
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Data Analysis 55To import the sampl
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Data Analysis 57Using Expected Frag
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Data Analysis 59Forcing Expected Pe
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Data Analysis 61Viewing the EFs/EPs
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Data Analysis 63Changing the Peak F
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Data Analysis 65Merging Two PeaksIf
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Data Analysis 67Using the Default L
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Data Analysis 69Upper and Lower Mar
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Data Analysis 71Lower Marker Peaks
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Data Analysis 73Viewing GelsTo comp
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Data Analysis 75Adjust Pane WidthsT
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Data Analysis 77Viewing Graphs in t
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Data Analysis 79Viewing Multiple Pr
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Data Analysis 81Reanalyzing a Data
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Data Analysis 83ElectropherogramCho
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Data Analysis 85Peak TableChoosing
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Data Analysis 87Raw DataASCII text
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Data Analysis 89Exporting Data Manu
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Software Security 91• “Backing
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Software Security 93Managing User A
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Software Security 95Changing User I
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Software Security 97Changing Access
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Software Security 99Setting Policie
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Software Security 101Electronic Sig
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Software Security 103Audit TrailLab
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Software Security 105Exporting the
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Software Security 107Creating New D
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Software Security 109Remote CDR Ser
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Software Security 111Backing Up Dat
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Software Reference 113Software Refe
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Software Reference 115Menu BarThe m
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Software Reference 117Collection Me
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Software Reference 119Security Menu
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Software Reference 121Chip Status a
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Software Reference 123Error Message
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Software Reference 125Rename Plate
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Software Reference 127Collection Pa
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Software Reference 129Graph View (C
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Software Reference 131Active Data T
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Software Reference 133Electropherog
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Software Reference 135OptionText Or
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Software Reference 137Gel View (Con
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Software Reference 139Gel View Prop
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Software Reference 141Peak Table Vi
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Software Reference 147Filter ViewTh
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Software Reference 149Filter View (
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Software Reference 151Add New Expec
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Software Reference 153Assay Analysi
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Software Reference 155Alignment Tab
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Software Reference 157Analysis Tab
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Software Reference 159Peak Find Tab
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Software Reference 161Table 2. Well
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Software Reference 163Expected Frag
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Software Reference 165Excluded Peak
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Software Reference 167Smear Analysi
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Software Reference 169Titer Tab (Co
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Software Reference 171Advanced TabU
Page 173 and 174: Software Reference 173Audit Trail W
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Page 177 and 178: Software Reference 177DeleteButtonH
Page 179 and 180: Software Reference 179CDR Utility W
Page 181 and 182: Software Reference 181Change Passwo
Page 183 and 184: Software Reference 183Event Viewer
Page 185 and 186: Software Reference 185Export Window
Page 187 and 188: Software Reference 187Installation
Page 189 and 190: Software Reference 189Login WindowU
Page 191 and 192: Software Reference 191Perform Signa
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Page 195 and 196: Software Reference 195Print Validat
Page 197 and 198: Software Reference 197Run File Edit
Page 199 and 200: Software Reference 199Sample Name E
Page 201 and 202: Software Reference 201Save Workspac
Page 203 and 204: Software Reference 203Start Run Win
Page 205 and 206: Software Reference 205Run Tab (Cont
Page 207 and 208: Software Reference 207Output Tab (C
Page 209 and 210: Software Reference 209Advanced Tab
Page 211 and 212: Software Reference 211System Diagno
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Page 215 and 216: Software Reference 215Edit UsersUse
Page 217 and 218: Software Reference 217De/Activate U
Page 219 and 220: Software Reference 219Show/Hide in
Page 221 and 222: LabChip GX Instrument Description 2
Page 223: LabChip GX Instrument Description 2
Page 231 and 232: Maintenance and Service 231Maintena
Page 233 and 234: Troubleshooting and Diagnostics 233
Page 247 and 248: Glossary of Terms 247Glossary of Te
Page 249 and 250: Glossary of Terms 249CLAThe file ex
Page 251 and 252: Glossary of Terms 251Data Filtering
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Page 255 and 256: Glossary of Terms 255Figure 115. Sl
Page 257 and 258: Glossary of Terms 257Ladder Analysi
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Page 261 and 262: Glossary of Terms 261Polynomial Ord
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Page 265 and 266: Glossary of Terms 265SmearSpecifies
Page 267 and 268: Glossary of Terms 267Figure 120. Pr
Page 269 and 270: Glossary of Terms 269WorkspaceZero
Page 271 and 272: Caliper Life Sciences, Inc. Product
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Index 275IndexNumerics21 CFR Part 1
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Index 277showing. . . . . . . . . .
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Index 279clearing default ladder .
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Index 281Remote CDR Server . . . .
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Index 283Waste wells . . . . . . .
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LabChip GX/GXII User Manual

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