ICS-1000 Ion Chromatography System Operator's Manual

ICS-1000 Ion Chromatography System5.1.2 Calibrating the Conductivity CellWhen to Calibrate• After every 6 months of useNOTEDo not use this procedure to calibrate a new cell. If youare replacing a cell, see the instructions in Section 5.9 toinstall the new cell and calibrate it.Items NeededDescription1.0 mM KCl solution Prepare by dissolving 0.07456 g of reagentgradeKCl in 1 liter of 18-megohm DIwater.Backpressure tubing to provideat least 7 MPa (1000 psi)Use 0.076-mm (0.003-in) ID yellow PEEKtubing (P/N 049715).1. Open the ICS-1000 Wellness Panel in Chromeleon (seeSection 5.1.1).2. On the Wellness Panel under Electric Conductivity CellCalibration, click Offset Cal.3. When the offset calibration is complete, click Slope Cal on theWellness Panel.4. Disconnect the pump output line from the injection valve.5. Disconnect the line from the suppressor ELUENT OUT port to the cellinlet and connect the pump output line directly to the cell inlet.6. Verify that there is a minimum of 7 MPa (1000 psi) of backpressure.7. On the Wellness Panel under Conductivity Cell Calibration, clickCell 35 C. Allow the cell to reach this temperature and then wait anadditional 5 minutes to let it stabilize.8. Select 1.00 mL/min to begin pumping 1.0 mM KCl through the cell at1.0 mL/min.9. Wait until the total conductivity reading stabilizes (in about15 minutes) and then select Calibrate on the Wellness Panel.5-4 Doc. 031879-01 3/03