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Operational Plan for the Restoration of Diadromous Fishes to the ...

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designs (GRTS), where samples are regularly distributed in space with known<br />

inclusion probabilities. Such samples are compatible with standard statistical<br />

methods (e.g. parametric and non-parametric methods), but statistical methods that<br />

take advantage <strong>of</strong> inclusion probability may also be used, such as those in <strong>the</strong> R<br />

package psurvey.analysis. We used <strong>the</strong> Penobscot Basin USGS NHD High GIS<br />

data as our sampling frame, and developed sampling designs in R. These designs<br />

can be readily edited <strong>to</strong> accommodate alternative sampling needs. Fur<strong>the</strong>r,<br />

although we focused only on <strong>the</strong> Penobscot <strong>for</strong> this exercise, we will create a statewide<br />

sampling design that integrates <strong>the</strong> Penobscot sampling plan. This will allow<br />

<strong>the</strong> same level <strong>of</strong> statistically rigorous sampling within <strong>the</strong> Penobscot basin, but also<br />

allow <strong>the</strong> Penobscot data and all o<strong>the</strong>r data within <strong>the</strong> state-wide sampling plan <strong>to</strong> be<br />

integrated. A final sampling plan <strong>for</strong> <strong>the</strong> Penobscot should be integrated in<strong>to</strong> a<br />

larger DPS-wide plan, and development <strong>of</strong> <strong>the</strong> plan should follow <strong>the</strong> steps outlined<br />

later in this document.<br />

For juvenile survey, we recommend a mix <strong>of</strong> full population estimates and relative<br />

abundance estimates per<strong>for</strong>med by electr<strong>of</strong>ishing. Fur<strong>the</strong>rmore, additional panels<br />

could be constructed <strong>to</strong> <strong>for</strong>mally include CPUE and snorkel samples.<br />

For redd surveys we recommend developing several index “reaches’ that are<br />

surveyed every year, preferably multiple times per year <strong>to</strong> ensure <strong>the</strong> most accurate<br />

counts possible, and a multi-panel GRTS design <strong>to</strong> provide greater spatial coverage.<br />

Process<br />

The final sampling plan should follow <strong>the</strong> process outlined in Figure 1. The plan will<br />

be constructed <strong>to</strong> address <strong>the</strong> various sampling needs <strong>to</strong> meet <strong>the</strong> objectives<br />

defined in <strong>the</strong> sample design process.<br />

Figure 1. Flowchart <strong>of</strong> sampling design development<br />

source: http://www.epa.gov/nheerl/arm/designpages/designprocoverview5.htm<br />

PRFP Page 191

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