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Operational Plan for the Restoration of Diadromous Fishes to the ...

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The process begins with defining <strong>the</strong> objectives <strong>of</strong> <strong>the</strong> sampling program, and <strong>the</strong>n<br />

defines <strong>the</strong> target population(s) and <strong>the</strong> attribute(s) that are <strong>of</strong> interest. Next, <strong>the</strong><br />

sampling frame requirements and materials are specified, as are <strong>the</strong> sampling<br />

design requirements <strong>to</strong> meet <strong>the</strong> objectives. A sampling design and final sampling<br />

frame are created, and <strong>the</strong> design is <strong>the</strong>n used <strong>to</strong> select sampling sites from <strong>the</strong><br />

frame. The results are output as an ESRI shapefile with an attribute table. Overall,<br />

this must be an iterative process, balancing various sampling objectives with<br />

achievable sampling ef<strong>for</strong>t. There will be a trade<strong>of</strong>f between sampling <strong>for</strong> status and<br />

trends verses sampling <strong>to</strong> test specific hypo<strong>the</strong>ses. The objectives must balance<br />

<strong>the</strong>se competing needs given a certain amount <strong>of</strong> ef<strong>for</strong>t. The sample design should<br />

also address both short and long term needs. Sampling <strong>to</strong> assess particular<br />

management actions may require a very different design than that needed <strong>to</strong> asses<br />

naturally reproducing populations.<br />

Sample Size <strong>for</strong> Electr<strong>of</strong>ishing<br />

Sample size and power present many challenges. We have chosen <strong>to</strong> sample at<br />

least 5-10 sites per drainage statewide based on <strong>the</strong> analyses described below<br />

(also, see Figure 2):<br />

“We recommend a minimum <strong>of</strong> 5 – 10 sites be sampled annually within a HUC <strong>of</strong><br />

interest using ei<strong>the</strong>r multiple pass removal estimates <strong>of</strong> mean density or mean<br />

CPUE methodologies. This amount <strong>of</strong> sampling ef<strong>for</strong>t will provide 80% power in<br />

detecting a trend in <strong>the</strong> index <strong>of</strong> abundance <strong>for</strong> annual rates <strong>of</strong> change between 0.1<br />

and 0.2. Although <strong>the</strong> ability <strong>to</strong> detect smaller changes is desirable, <strong>the</strong> amount <strong>of</strong><br />

sampling required <strong>to</strong> detect such changes greatly increases at annual rates <strong>of</strong><br />

increase less than 0.1 and may not be feasible with limited sampling resources.<br />

Annual rates <strong>of</strong> change <strong>of</strong> 0.1 <strong>to</strong> 0.2 correspond <strong>to</strong> approximately a doubling or<br />

tripling <strong>of</strong> <strong>the</strong> index <strong>of</strong> abundance in a 10 year period. Because <strong>of</strong> <strong>the</strong> natural annual<br />

variation in parr abundance, anything less than a true doubling <strong>of</strong> abundance may<br />

be <strong>of</strong> little <strong>to</strong> no significance in overall population growth rates <strong>of</strong> Atlantic salmon.”<br />

(from J. Sweka, J. Trial, and J. Kocik, draft White Paper on Atlantic Salmon S<strong>to</strong>ck<br />

Assessment Status and Needs, April 7, 2008.).<br />

While trend detection is one goal <strong>of</strong> juvenile assessment, o<strong>the</strong>r goals such as<br />

describing <strong>the</strong> status <strong>of</strong> juvenile abundance, or mechanistic relationships between<br />

environmental variables and juvenile abundance, survival, or growth, are critically<br />

important. There<strong>for</strong>e, <strong>the</strong> sampling we describe here is a minimum <strong>to</strong> detect trends<br />

and describe status, but more in depth sampling may be required <strong>for</strong> some<br />

objectives.<br />

The 5-10 sites will represent annually sampled sites where full population estimates<br />

are per<strong>for</strong>med. In addition, a rotation <strong>of</strong> sites that includes CPUE and population<br />

estimates will be sampled. The design <strong>of</strong> this will be specific <strong>to</strong> <strong>the</strong> drainage, but will<br />

be based on a three year rotation, where each site will be visited every third year,<br />

PRFP Page 192

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