IARC MONOGRAPHS ON THE EVALUATION OF CARCINOGENIC ...

Table 3. DNA adduct formation in Chinese herb nephropathy (CHN) patients
Details of study DNA binding Reference
Detection of AA-specific DNA adducts in renal tissue from 5 female patients
with CHN from Belgium by 32 P-postlabelling. DNA was obtained from
cortical or cortico-medullary tissue. The patients had taken pills containing
Chinese herbs for 13–23 months. The adduct chromatographically
indistinguishable from dA-AAI was detectable up to 27 months after
termination of the regimen.
Detection of AA-specific DNA adducts in the kidneys and one ureter from 6
female patients with CHN from Belgium by 32 P-postlabelling (five cases
were from Schmeiser et al., 1996). Three AA-specific adducts
chromatographically indistinguishable from dA-AAI, dG-AAI and dA-AAII
were detectable up to 44 months after termination of the regimen.
Detection of AA-specific DNA adducts in the kidneys of CHN patients
(37 female and one male), and in 17 ureters from 11 of these patients from
Belgium by 32 P-postlabelling. Among these patients were 18 cases of
urothelial carcinoma. Data were related to cumulative doses of compounds
in the weight-reducing pills on the basis of all prescriptions made during the
period of exposure (1990–92). The cumulative dose of aristolochia was a
significant risk factor for developing urothelial carcinoma. The kidneys and
ureters from the CHN patients had the same pattern of adducts consisting of
dA-AAI, dG-AAI and dA-AAII. The major adduct dA-AAI was detectable
up to 89 months after discontinuation of use of the weight-reducing pills. No
statistically significant difference was observed between mean levels of dA-
AAI DNA adducts determined in renal tissue samples from patients who had
developed urothelial carcinoma and those from tumour-free patients.
Detection of AA-specific DNA adducts in the kidneys from 2 new female
CHN patients from the Belgian cohort by 32 P-postlabelling.
dA-AAI: 0.7–5.3/10 7 nucleotides Schmeiser
et al. (1996)
dA-AAI: 0.7–5.3/10 7 nucleotides
dG-AAI: 0.02–0.12/10 7 nucleotides
dA-AAII: 0.06–0.24/10 7 nucleotides
In the kidneys: dA-AAI: 1.2–165/10 9 nucleotides
dG-AAI: 0.4–8.2/10 9 nucleotides
dA-AAII: 0.6–6.8/10 9 nucleotides
In the ureters: dA-AAI: 2.2–34/10 9 nucleotides
Bieler et al.
(1997)
Nortier et al.
(2000)
2.9–5.0/10 8 nucleotides Arlt et al.
(2001a)
SOME TRADITIONAL HERBAL MEDICINES 97