IARC MONOGRAPHS ON THE EVALUATION OF CARCINOGENIC ...

Table 16. Genetic and related effects of aflatoxin B1
Test system
Result a
Without
exogenous
metabolic
system
With
exogenous
metabolic
system
Dose b
(LED or HID)
Reference
Salmonella typhimurium TA100, TA98, reverse mutation NT + 2.5 ng/tube Loarca-Piña et al.
(1996)
Salmonella typhimurium TA100, TA98, reverse mutation + NT 0.6 μg/tube Loarca-Piña et al.
(1998)
Salmonella typhimurium TA98, reverse mutation (S9 mix from HepG2 cells) NT + 2 μg/plate Knasmüller et al. (1998)
Saccharomyces cerevisiae with recombinant CYP1A2 (human), mitotic
recombination
+ NT 5 Kaplanski et al. (1998)
DNA damage (comet assay), human HepG2 cells in vitro + 0.0025 Uhl et al. (2000)
Gene mutation, human hepatoma (HepG2) cells in vitro, HPRT locus NT + 0.5 Knasmüller et al. (1998)
Gene mutation, Chinese hamster ovary AS52 cells in vitro, Gpt locus NT + 0.16 Goeger et al. (1998)
Gene mutation, Chinese hamster ovary K1BH4 cells in vitro, Hprt locus NT + 0.31 Goeger et al. (1998)
Gene mutation, Chinese hamster ovary K1BH4 cells in vitro, Hprt locus NT + 0.31 Goeger et al. (1999)
Gene mutation, mouse lymphoma L5178Y cells in vitro, tk locus NT + d
0.005 Preisler et al. (2000)
Recombination, yeast S. cerevisiae in vitro c
+ NT 7.8 Sengstag et al. (1996)
Recombination, Chinese hamster V79 SP5 cells in vitro, reversion mutation
assay
NT + 0.19 Zhang & Jenssen (1994)
Micronucleus formation, Chinese hamster V97MZr2B1 cells in vitro e
+ NT 0.031 Reen et al. (1997)
Cell transformation, rat liver epithelial BL9 cells – + 7.5 Stanley et al. (1999)
Gene mutation, human lymphoblastoid cells (recombinant CYP1A1) in vitro,
HPRT locus
+ NT 0.004 Cariello et al. (1994)
Recombination, human lymphoblastoid TK6 cells in vitro, TK locus NT + 0.016 Stettler & Sengstag
(2001)
Sister chromatid exchange, human lymphocytes in vitro + + 9.4 Wilson et al. (1995)
Sister chromatid exchange, human leukocytes in vitro NT + f
0.31 Wilson et al. (1997)
AFLATOXINS 235