IARC MONOGRAPHS ON THE EVALUATION OF CARCINOGENIC ...

Table 8 (contd)
Test system
Drosophila melanogaster, somatic mutations, recombination, sexlinked
recessive mutation
Result a
Without
exogenous
metabolic
system
With
exogenous
metabolic
system
Compound,
dose (LED or HID) b
+ NT AA mixture (65% AAI,
35% AAII), 17 μg/mL in
feed
Reference
Frei et al. (1985)
DNA strand breaks, rat hepatocytes, in vitro (alkaline elution assay) – NT AAI and AAII [dose not
reported]
Pool et al. (1986)
Gene mutation, rat subcutaneous granuloma tissue, Hprt locus in vitro + NT AAI and AAII, 20 Maier et al. (1987)
Gene mutation, Chinese hamster ovary cells, Hprt locus in vitro + NT AAI, 18.2 Pezzuto et al. (1988)
Sister chromatid exchange, human lymphocytes in vitro + NT AA mixture, 1.0 Abel & Schimmer
(1983)
Micronucleus induction, human lymphocytes and hepatoma cells + + AA mixture, 17 Kevekordes et al.
(Hep-G2) in vitro
(2001)
Chromosomal aberrations, human lymphocytes in vitro + NT AA mixture, 1.0 Abel & Schimmer
(1983)
Unscheduled DNA synthesis, male F344/Ducrj rat glandular stomach
mucosa in vitro, after in-vivo treatment
– AA mixture, 400 × 1 po Furihata et al. (1984)
Unscheduled DNA synthesis, male PV6 rat glandular stomach
mucosa in vivo
– AA mixture, 300 × 1 po Burlinson (1989)
Gene mutation, male SD rat subcutaneous granuloma tissue,
+ AA mixture, 40 μg in Maier et al. (1985)
Hprt locus in vivo
pouch
Gene mutation, male SD rat subcutaneous granuloma tissue,
+ AAI, 80 μg; AAII, 32 μg Maier et al. (1987)
Hprt locus in vivo
in pouch
Micronucleus induction, male and female NMRI mouse bone-marrow
cells in vivo
+ AA mixture, 20 × 1 iv Mengs & Klein (1988)
AA, aristolochic acids (mixed); AAI and AAII, aristolochic acids I and II
a +, positive; (+), weak positive; –, negative; NT, not tested
b LED, lowest effective dose; HID, highest ineffective dose; in-vitro tests, μg/mL; in-vivo tests, mg/kg bw/day; iv, intravenous; po, oral
c Activity is much less in the presence of metabolic activation.
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