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Resource Book on Horticulture Nursery Management

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30-45 cm and <strong>on</strong>e to two shoots are retained. The plants are placed in the 15-20 cm deep<br />

pit and pressed firmly to avoid the air space. The planted rootstock sprout in 20-30 days.<br />

They produce newer shoots from base and these are utilized for budding.<br />

Budding of Rootstock in Roses:<br />

The budding procedure c<strong>on</strong>sists of making a vertical and horiz<strong>on</strong>tal cut in the<br />

rootstock to form a “T”. The T is placed well below the shoot that arises from the<br />

rootstock. Cuts are made <strong>on</strong>ly to the depth of cambium layer. An eye is removed from the<br />

marked cultivar making a shallow slicing cut to form a shield like piece as backing for<br />

the bud. It is inserted between the flaps formed by the bark <strong>on</strong> either side of the T. A<br />

budding tape is wrapped around the shank of the stock above and below the eye to hold it<br />

in place. The budding operati<strong>on</strong>s are completed by the 15 th February.<br />

Three to four weeks after budding, the rootstock is cut approximately <strong>on</strong>e-third of<br />

the way through directly above the inserted bud and top is broken over. This places the<br />

bud in an apical positi<strong>on</strong> <strong>on</strong> the shank of the rootstock, where it begins to grow. Three<br />

weeks after the tops are broken over they are removed entirely from the plant. Top<br />

removal is d<strong>on</strong>e in two stages to prevent the complete defoliati<strong>on</strong> at any time. The<br />

digging of the budded plants is carried out in mid of October and completed by the end of<br />

January.<br />

Budded Rose<br />

In Vitro Propagati<strong>on</strong> of Roses:<br />

In vitro propagati<strong>on</strong> of roses has played a very important role in rapid multiplicati<strong>on</strong><br />

of cultivars with desirable traits and producti<strong>on</strong> of healthy and disease-free plants. During<br />

the last several years, different approaches have been made for in vitro propagati<strong>on</strong> of<br />

roses. Micropropagati<strong>on</strong> is d<strong>on</strong>e using apical buds or nodal segments. It is very<br />

necessary to study the specific nutriti<strong>on</strong>al requirements of the cuttings at different growth<br />

<str<strong>on</strong>g>Resource</str<strong>on</strong>g> <str<strong>on</strong>g>Book</str<strong>on</strong>g> <strong>on</strong> <strong>Horticulture</strong> <strong>Nursery</strong> <strong>Management</strong> : 156

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