Final version of Tropical Legumes II Project Report for Phase 1 - icrisat

More documents

Recommendations

Info

Table 5-11: Ten SNPs converted and used for the validation Primer CHR Posn Trait SNP Name Primer (cM) AS36 LG_03 1.3 CoBB-1 UP12_4270_135 F-CCTCTTCGCAGCCGCATTATCTCT R-CGTGCCGCTCGTGCCATAGTC ASS6 LG_03 0 CoBB-1/ *CPMVnewb UP12_12905_686 F-CTGGACACAGTGGGTATGTGAACACTGAC R-ATAACCACTAAACAAAGTGCTTCCATCTGCACT ASS93 LG_03 72.4 DLS-1/2 UP12_17547_561 F-CCAAACTTGGGGCTCGTCTCAGC R-TGTCAATGATGATCTTCCTCCCGGTC ASS16 LG_03 1.9 Macrophomina UP12_9955_544 R-AGAAGAGTGGAAGCGGCGATGGAT F-ATGACTCTTCTCTCAAATATTGAGAAGTTCATTTCT ASS64 LG_03 38.7 SUR UP12_13864_1798 F-TCACTCCGTTCCTAAGGTTCGGCC R-TTTACCGTTACAAACAAACACAAGCTCCTTAGTG ASS39 LG_07 15.8 Drought UP12_998_587 F-GAGAAAAGCTTAATTAAACCGAAACAGGCG R-AGAAGAAGACCTATACCAAGCCTAAGAAGATCAAGC AS100 LG_08 0 Drought UP12_14702_888 F-CCGCCTAGAGTTCCACGCTTATCG R-GTGGTGTGTTAGGTTGTGAGAGGAAGAGTGA ASS110 LG_08 22.9 Gy-1 UP12_5650_935 F-TGCAATGTTAGTGGCATTGTACTGGGG R-TAAACCATGCTAAGAATGGCGTCGACA ASS130/ AS113 LG_09 7.3 CoBB-3 UP12_5698_563 F-ATCAAGTAAACAAAACAAAAACAGCCAGAATATATG R-CTCAGAAGCACCCAAATACAAGCTCATCA ASS133 LG_10 39 Striga UP12_12584_1346 F-TTGTAGCTGCTATTTTAAGAAAGCTAACAACCTCC F-CATGAATTGGAGCCAACTAAAACAACCAAA Marker validation: In this study the feasibility of applying the designed SNP markers with 32 samples from a cowpea breeding population were tested. All the AS-PCR primer sets selected were optimized and validated. Ten SNPs, potentially linked to CoBB-1,*CPMVnewb, DLS-1/2, Macrophomina, SUR, Drought, Gy-1, CoBB-3 and Striga were converted to agarose gel assay. Information about SNP genotype was provided by the presence (scored as 1) or absence (scored as 0) of the PCR amplification product from allele specific primers (Figure 5, Table 9) 102 Engaging Smallholder Farmers | Tropical Legumes II Project
ASS6 ASS64 ASS110 ASS56 Figure 5-9: Examples of AS-PCR mapping results The identified SNPs from cowpea ESTs linked to important traits have been converted into genetic markers. The converted SNPs are covering several regions of the cowpea genetic map, LG 3 (0 ~ 1.3cM), LG 7 (13~17.8 cM), LG8 (0~1.3cM), LG9 (3.4~7.3cM), and LG10 (40.8~41.7cM), where genes for several important traits may be present. Allele specific PCR primers (AS-PCR) were designed and tested with a cowpea breeding population. Out of the 57 SNP targeted using mismatch approach, amplification for 10 SNP were found to give robust AS-PCR, which is a good success rate for the marker development. Allele specific amplification was observed for at least one of the two alternative alleles at these SNP sites. In this study we found AS-PCR to be an efficient, cost effective and reliable way for SNP validation. Earlier studies in barley also revealed efficient validation of SNPs by allele specific PCR. Progress of Phase 1 103
Page 1 and 2:
Tropical Legumes II Project TROPICA
Page 3:
TROPICAL LEGUMES II Tropical Legume
Page 6 and 7:
Seed Production and Delivery System
Page 8 and 9:
Seed production and delivery system
Page 10 and 11:
NARES NASFAM NASSPA NPT NSC NSO NSS
Page 12 and 13:
TL II was implemented in 10 target
Page 14 and 15:
country governments in agricultural
Page 16 and 17:
Table 1-2: continued Variety code (
Page 18 and 19:
Table 1-3: Seed production and deli
Page 20 and 21:
As stated earlier in this chapter,
Page 22 and 23:
Table 1-5: Graduate degree students
Page 24 and 25:
Ndjeunga J, Ibro A, Cisse Y, Ben Ah
Page 26 and 27:
Project background Groundnut is one
Page 28 and 29:
Table 2-2: Performance of ICGV 0035
Page 30 and 31:
Table 2-6: Performance of ICGV 9111
Page 32 and 33:
Table 2-11: Results of 255 paired c
Page 34 and 35:
Table 2-16: Groundnut seed of vario
Page 36 and 37:
Infrastructure facilities For effic
Page 38 and 39:
Project sites Tamil Nadu The presen
Page 40 and 41:
In Karnataka, the PDKV model was pr
Page 42 and 43:
Training course A total of four tra
Page 44 and 45:
Background The yield of groundnut i
Page 46 and 47:
Table 3-2: Groundnut production tre
Page 48 and 49:
In order of importance, the largest
Page 50 and 51:
Table 3-5: Results of pair-wise com
Page 52 and 53:
Finally, the variety choices are co
Page 54 and 55:
hybridization techniques, design of
Page 56 and 57:
Figure 3-2: The Malawi model of the
Page 58 and 59:
Tanzania TL II has contributed to t
Page 60 and 61:
Annex 3-2: Performance of Virginia
Page 62 and 63: Annex 3-4: Regional elite groundnut
Page 64 and 65: 54 Engaging Smallholder Farmers | T
Page 66 and 67: The European Union still remains th
Page 68 and 69: Table 4-2: Proportion of households
Page 70 and 71: Table 4-4: Amount requested, amount
Page 72 and 73: Table 4-6. Comparison of groundnut
Page 74 and 75: The use of other inputs (credit, in
Page 76 and 77: A PVS evaluation was launched in pi
Page 78 and 79: Table 4-10: Locations and contact f
Page 80 and 81: Table 4-11: Mean pod yield (kg per
Page 82 and 83: 99511 (Table 4-12). Most important
Page 84 and 85: Table 4-15: Evaluation of groundnut
Page 86 and 87: A 10-day intensive training worksho
Page 88 and 89: Seed production of all classes A to
Page 90 and 91: Testing seed marketing strategies A
Page 92 and 93: Area Global trends in area under co
Page 94 and 95: Figure 5-3 shows that Nigeria made
Page 96 and 97: much greater deficits. Under this s
Page 98 and 99: and other inputs. • Popular varie
Page 100 and 101: Mozambique Soybean seed multiplicat
Page 102 and 103: dry savanna regions, where the bulk
Page 104 and 105: Number of demonstration plots in ea
Page 106 and 107: Mozambique Many farmers have shown
Page 108 and 109: Table 5-7: Number of days to flower
Page 110 and 111: most efficient and IT99K-7-21-2-2 w
Page 114 and 115: Strengthening capacity of national
Page 116 and 117: Infrastructure development at NARS
Page 118 and 119: in Mali, 107.3 MT in Mozambique, an
Page 120 and 121: produced over the first phase of th
Page 122 and 123: Lessons learned TL II cowpea seed s
Page 124 and 125: Phase I Social Sciences research in
Page 126 and 127: The consequences are that the impro
Page 128 and 129: about half of area share of land al
Page 130 and 131: Figure 6-3: Average area (ha) per h
Page 132 and 133: single lingering bottleneck on whic
Page 134 and 135: In Kenya, the small seed pack appro
Page 136 and 137: Summary, process gains and people r
Page 138 and 139: Fast-Tracking and Technology Genera
Page 140 and 141: Figure 6-6: Distribution of PVS sit
Page 142 and 143: Table 6-12: Yields of fast track bu
Page 144 and 145: Table 6-15: Farmer evaluation of in
Page 146 and 147: While the fast track entries were r
Page 148 and 149: Technology generation Mechanistic s
Page 150 and 151: 60 50 Rainfall Pan evaporation Maxi
Page 152 and 153: 16, and the advantage of some ALB l
Page 154 and 155: Number of degree training students
Page 156 and 157: Number of NARS technicians trained
Page 158 and 159: Rain-out shelter in CIAT-Colombia T
Page 160 and 161: Impact assessment and enhancement P
Page 162 and 163:
Annex 6-2: TL IIEthiopia: decentral
Page 164 and 165:
154 Engaging Smallholder Farmers |
Page 166 and 167:
farmers for enhancing their awarene
Page 168 and 169:
• Designing, testing and implemen
Page 170 and 171:
Andhra Pradesh The overall sample s
Page 172 and 173:
Prakasam district: Crops grown in P
Page 174 and 175:
Constraints in adoption of chickpea
Page 176 and 177:
Cost-benefit analysis for chickpea
Page 178 and 179:
Identification of farmer-and-market
Page 180 and 181:
Table 7-6: Breeder Seed of differen
Page 182 and 183:
Capacity building Knowledge empower
Page 184 and 185:
Table 7-15: Training of local seed
Page 186 and 187:
• In addition to yield, maturity
Page 188 and 189:
Introduction Chickpea is grown in E
Page 190 and 191:
Chickpea seed access The first majo
Page 192 and 193:
Grades and standards About 75% of t
Page 194 and 195:
Table 8-6: Varieties used in PVS tr
Page 196 and 197:
private sector lacks the incentive
Page 198 and 199:
• LZARDI-Ukiriguru: Land along wi
Page 200 and 201:
• Establishing functional legume
Page 202 and 203:
Table 9-1: Farmers’ preferred var
Page 204 and 205:
Table 9-3: Baby trials conducted in
Page 206 and 207:
Table 9-5: Reaction of pigeonpea li
Page 208 and 209:
TL II focuses on farmer-participato
Page 210 and 211:
Production of Breeder, Foundation,
Page 212 and 213:
Mahaboobnagar and Ranga Reddy distr
Page 214 and 215:
(Table 9-9 Continued) Particular To
Page 216 and 217:
Chichewa) and Tanzania (Swahili). O
Page 218 and 219:
Available technologies Although imp
Page 220 and 221:
and Chitedze in Malawi showed that
Page 222 and 223:
Table 10-6: Various classes of qual
Page 224 and 225:
Awareness activities Farmers’ fie
Page 226 and 227:
Annex 10-1: Target locations for pi
Page 228 and 229:
Adoption studies Adoption studies f
Page 230 and 231:
3.5 Yields (Tonnes/hectare) 3.0 2.5
Page 232 and 233:
Supply of important traits by the i
Page 234 and 235:
and marketing organization in Malaw
Page 236 and 237:
Table 11-3: Grain and fodder yields
Page 238 and 239:
Table 11-9: Communities and famers
Page 240 and 241:
Table 11-14 Average performance of
Page 242 and 243:
Page 244 and 245:
Table 11-16: Soybean Breeder’s Se
Page 246 and 247:
Table 11-19: Grain yield (kg per ha
Page 248 and 249:
Table 11-23: F3 Segregating populat
Page 250 and 251:
At TSBF-CIAT in Kenya, a study was
Page 252 and 253:
Number of NARS scientists trained T
Page 254 and 255:
Improved access to markets Improved
Page 256 and 257:
Total 107.3 259.0 609.0 975.3 246 E
Page 258 and 259:
utilization and marketing. In Niger
Page 260 and 261:
Annex 11-2: Tobit Model estimate of
Page 262:
show all

Final version of Tropical Legumes II Project Report for Phase 1 - icrisat

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?