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Final version of Tropical Legumes II Project Report for Phase 1 - icrisat

Final version of Tropical Legumes II Project Report for Phase 1 - icrisat

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Table 4-15: Evaluation <strong>of</strong> groundnut genotypes <strong>for</strong> their resistance to aflatoxin contamination, Samanko, Mali, 2008<br />

Genotype Pod yield (MT per ha) Aflatoxin content (ppb)<br />

ICGV 94379 2686 1.36<br />

ICGV 94434 2489 37.18<br />

ICGV 91278 1801 5.11<br />

ICGV 91328 2113 8.17<br />

ICGV 91341 2167 82.37<br />

ICGV 93305 2794 0.89<br />

ICGV 91317 2081 2.47<br />

ICGV 91279 2149 0.97<br />

ICGV 91284 1769 1.69<br />

ICGV 92302 1674 2.01<br />

ICGV 91315 1987 1.80<br />

ICGV 91324 2087 1.44<br />

ICGV 93328 1132 8.76<br />

ICGV 91283 523 1.80<br />

ICGV 89104 2039 2.12<br />

55-437 (Resistant check) 1690 1.23<br />

JL 24 (susceptible check) 1917 899.12<br />

SEM (±) 156 4.67<br />

CV (%) 14 23<br />

RCBD design with 3 replications; plot size = 12 m 2<br />

New breeding populations<br />

Generation advance<br />

Sixty-nine F 3<br />

and 28 F 5<br />

bulk populations were grown at ICRISAT-Mali <strong>for</strong> generation advance. Over<br />

600 individual plant selections were made based on pod load and other desirable agronomic<br />

characteristics. The seed <strong>of</strong> each single plant was increased in the 2009 <strong>of</strong>f-season under irrigation.<br />

These were evaluated <strong>for</strong> agronomic traits in the main crop season.<br />

Generation <strong>of</strong> new breeding populations<br />

Fifty three crosses (30 <strong>for</strong> foliar diseases resistance, 12 <strong>for</strong> productivity, 10 <strong>for</strong> aflatoxin contamination<br />

tolerance, and 1 <strong>for</strong> limited fresh seed dormancy) were made between September and December 2008<br />

to generate populations with the desired attributes. Parental lines used included the most popular<br />

varieties in West Africa and high yielding foliar diseases resistant lines ICG # 7878, 7, 6222, 4440, and<br />

ICG (FDRS) 4. In the productivity crosses, the new parental lines were ICGV 00350 and ICGV 9114.<br />

For aflatoxin, the sources <strong>of</strong> resistance/tolerance are 55-437 and J11.<br />

In the 2009 crop season, the 53 F1 <strong>of</strong> the crosses made in the previous season were sown at ICRISAT-<br />

Mali to produce the F2. Fifteen new crosses involving farmers- and market-preferred traits as parents<br />

were completed to generate new populations with enhanced productivity.<br />

In 2010, 59 F 2:3<br />

populations involving the most popular varieties in West Africa and sources <strong>of</strong> resistance<br />

to foliar diseases, aflatoxin contamination and rosette were advanced to the next generation. Population<br />

size varied from 200 to 800 plants per population. Single plants and bulks were selected. Five <strong>of</strong> these<br />

populations involving a stable source <strong>of</strong> ELS resistance (ICG 7878) crossed with the most popular<br />

varieties (55-437, 47-10, ICGV 86124 and Fleur 11) were advanced through single seed descent (SSD)<br />

during the post-rainy season (November-April) to produce F 4:5<br />

RILS <strong>for</strong> phenotyping in 2011 crop season<br />

(June-October). The stage is now set <strong>for</strong> developing populations to validate the QTLs <strong>for</strong> Marker assisted<br />

breeding and this shows a clear linkage between TL I and TL <strong>II</strong> projects.<br />

74<br />

Engaging Smallholder Farmers | <strong>Tropical</strong> <strong>Legumes</strong> <strong>II</strong> <strong>Project</strong>

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