Pesticide residues in food — 2007: Toxicological ... - ipcs inchem

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Table 7. Results of studies of genotoxicity with azinphos-methyl
End-point Test object Concentration Purity (%) Results Reference
In vitro
Reverse mutation
(Ames)
Reverse mutation
(Ames)
Reverse mutation
(Ames)
Reverse mutation
(Ames)
Reverse gene
mutation
DNA damage
Unscheduled
DNA synthesis
Clastogenicity
Sister chromatid
exchange
In vivo
Micronucleus
formation
Chromosomal
aberration
Dominant lethal
mutation
S. typhimurium TA98,
TA100, TA1535,
TA1537, TA1538
S. typhimurium TA98,
TA100, TA1535,
TA1537
S. typhimurium TA98,
TA100, TA1535,
TA1537
S. typhimurium TA98,
TA100, TA1535,
TA1537, TA1538
S. cerevisiae S138 and
S211α
E. coli W3110, E. coli
p3478
Rat hepatocytes
Chinese hamster ovary
cells-(K1)
Human lymphocytes
Chinese hamster lung
cells (V79)
Mouse
Rat, femoral bone
marrow
Mouse
0–160 μg/plate NS Negative Evanchik
(1987)
4–2500 μg/plate 92.3 Negative Herbold
(1978)
75–9600 μg/plate 92.5 Negative Herbold
(1988)
33–4000 μg/plate 88.8 Negative Lawlor
(1987)
33.3–10000 μg/ml 91.1 Negative Hoorn
(1983)
625–10000 μg/plate 91.1 Negative Herbold
(1984)
0.25–50.3 μg/ml, in
DMSO
60–120 μg/ml Approx.
90
5–500 μg/ml (+S9)
1–100 μg/ml (–S9)
in DMSO
91.1 Negative Myhr
(1983)
Dose-related chromatid
breaks and
exchanges
91.9 Clastogenicity observed
at cytotoxic
concentrations
+S9. No effect −S9
Alam et al.
(1974)
Herbold
(1986)
5–25 μg/ml 98.7 Negative Chen et al.
(1982a)
2.5–20 μg/ml Chen et al.
(1982b)
2 × 2.5 and 2 × 5 mg/kg
bw single oral dose in
Cremophor/water
5 mg/kg bw, single IP
dose in Cremophor/water
6.28 mg/kg bw, single
oral dose in methylcellulose
0.125 or 0.25 mg/kg bw,
single IP dose in corn oil
1 × 4 mg/kg bw, single
oral dose in Cremophor/
water
92.3 Negative Herbold
(1979a)
92.2 Negative Herbold
(1995)
92 Negative Henderson
et al. (1988)
NS Negative Arnold
(1971)
92.3 Negative Herbold
(1979b)
DMSO, dimethyl sulfoxide; IP, intraperitoneal; NS, not specified; S9, 9000 × g supernatant from livers of rats.
and 3.73 mg/kg bw per day in males and 0.55, 1.54, and 4.87 mg/kg bw per day in females). Treated
males and females were paired, and dams allowed to rear litters to weaning. Additional treated males
were paired with untreated females.
AZINPHOS-METHYL 139–172 JMPR 2007