Pesticide residues in food — 2007: Toxicological ... - ipcs inchem

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Table 15. Relevant findings in a study of prenatal developmental toxicity in rats given DEA
by gavage
Finding
Dose (mg/kg bw per day)
0 5 25 100
Food consumption (g/day):
Days 6–11 23 22 21* 16**
Days 11–16 26 25 26 24
Body-weight gain (g):
Days 6–11 26.0 24.3 21.5* 10.7**
Days 11–16 39.7 39.8 37.3 34.5*
Net body-weight change (g) from day 6 37.1 31.9 27.2 29.5
No. of animals pregnant 23 23 22 24
Postimplantatation loss (mean) 0.6 0.8 0.9 1.1
Postimplantation loss (%) 4.0 5.1 6.3 8.8
Mean No. of live fetuses 14.2 14.5 14.2 12.6
Mean fetal weights (g), males/females 5.7/5.3 5.6/5.3 5.8/5.4 5.6/5.3
Skeletal examination (No. of fetuses/litters) 168/23 174/23 164/22 158/24
Fused sternebrae 1 and 2 a 2/2 1/1 2/2 32**/14**
Total skeletal anomalies a 5/5 5/5 5/5 41/18**
Poster. digit 5, proximal phalanx; absent ossification a 16/11 23/12 8/6 28*/16
Total skeletal variations a 168/23 173/23 163/22 158/24
From Marty (1992b)
DEA, deethyl-atrazine.
a
No. of fetuses/litters.
* p < 0.05; ** p < 0.01.
single dose at 250, 500, 2000, 3500 or 5050 mg/kg bw by gavage. Each group consisted of five males
and five female with the exception of the group at the lowest dose that consisted of five females. The
rats were observed for clinical signs and mortality for 14 days. The LD 50
s for males, females and both
sexes combined were 2290, 810 and 1240 mg/kg, respectively (Kuhn, 1991d).
In a short-term study of oral toxicity, which complied with GLP and the test guidelines of
OECD and US EPA, groups of 10 male and 10 female KFM-WIST rats were fed diets containing DIA
(purity, 97.4%) at a concentration of 0, 50, 500 or 2000 ppm, equal to 0, 4.6, 46.5 and 161.2 mg/kg bw
per day in males and 0, 4.6, 49.7 and 164.7 mg/kg bw per day in females, for 4 weeks.
There was no mortality. Clinical signs of restlessness were observed in the rats at the highest
dose during the fourth week of treatment. At 2000 ppm, food consumption was reduced by approximately
60% during the first week of treatment and by about 40% over the 4-week treatment period.
Body weights were significantly reduced at 500 ppm from the third week and from the second week
at 2000 ppm; after 4 weeks the body weights at 2000 ppm were reduced by 34% (males) and 28%
(females) when compared with rats in the control group. Slight changes in haematological and clinical
biochemistry parameters were observed in the groups at the intermediate and highest dose, with
indication of a compensated haemolytic anaemia in the group at the highest dose. Absolute and relative
thymus weights were reduced at 500 ppm in males and at 2000 ppm in both sexes. No treatmentrelated
histopathological findings were noted in the groups at the lowest and intermediate dose. At
2000 ppm, mineralized deposits were noted in the renal pelvis of four males and two females, and a
minimal erosion of the gastric mucosa was noted in one male.
ATRAZINE 37–138 JMPR 2007