Photonic crystals in biology

Poster Session, Tuesday, June 15
Theme A1 - B702
L-cystein go ld nanoparticles on silica surface for HPLC separation
Yana Elfimova 1 *, Irina Ananieva 1 , Aleksandr Majouga 1 , Nikolay Zyk 1 and Oleg Shpigun 1
1 Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia
Abstract-New nanohybrid material for HPLC based on gold nanoparticles supported on silica gel and stabilized by
L-cystein is synthesized. The possibility of separation of -blockers and amino acids in different modes of chromatography is
studied.
Gold nanoparticles play an important role in applied
science and technology because they exhibit unique
chemical and physical properties which depend on their
shape, size, and local environment [1]. Materials based on
gold nanoparticles are widely used in the field of chemistry,
biology, material science and physics. The dimensions of
gold nanoparticles allow using them for the creation of a
new class of separation materials for liquid chromatography.
We suggest new sorbent based on the gold nanoparticles
supported on silica gel and stabilized by L-cystein for the
separation of different organic compounds including
optically active compounds.
Optically active compounds attract great attention because
of their widespread application in many fields of science,
particularly in pharmaceutics. Compounds that have optical
isomers in many cases exhibit different pharmacological and
toxicological properties. This fact has stimulated the
application of enantiomerically pure products. -blockers
play an important role among optically active compounds.
-blockers are drugs that slow the heart rate, decrease
cardiac output, lessen the force with which the heart muscle
contracts and reduce blood vessel contraction. They do this
by blocking beta-adrenergic receptors in various parts of the
body. Another important class of optically active
compounds is amino acids. Amino acids
co-exist in biological liquids of living organisms though
D- and L-isomers play different roles. That is why the
problem of enantioseparation of different amino acids is
very actual nowadays. HPLC has many applications in the
fields of determination of amino acids. The commonly used
method performance is the determination of amino acids as
hydrophobic derivatives in reverse-phase mode on the
hydrophobic stationary phase. In the present time much
works in chiral separations have been directed to the
development of new chiral selectors.
Gold nanoparticles with average 10 nm size have been
synthesized by reduction H[AuCl 4 ] in an aqua solution by
sodium citrate, and then they have been modified by
L-cystein. Prepared nanoparticles have been adsorbed on
silica gel. The scheme of the synthesized stationary phase
with L-cystein as organic lidand is presented on Fig.1.
Obtained material has been tested for ability of
-blockers
and aminoacids. The best separation of aminopyridines was
achieved in the normal-phase mode using 90% hexane/10%
isopropanol as a mobile phase. The retention of
aminopyridines rises with the growth of investigating
compounds polarity because of the increasing of dipoledipole
interactions.
We studied t-blockers enantioseparation
in normal-phase and polar-organic modes of
chromatography. The best results were observed for
pindolol at use of a mobile phase – acetonitrile / methanol /
CH 3 COOH / triethylamine (60/40/0.2/0.2).
The separation of amino acids was performed in the ion
exchange variant of chromatography with
spectrophotometric detection at 210 nm. We used 0.05M
borate buffer (pH 7.7) as the mobile phase. Because a small
molecule of L-cystein was used as a chiral selector the best
enantioseparation was achieved for such a small molecules
of amino acids as leucine and valine. Chromatogram of
enantioseparation is presented on Fig.2.
Figure 2. Chromatogram enantioseparation of pindolol. Column
(100 . Mobile phase:
acetonitrile/methanol/CH3COOH/threeethylamine (60/40/0,2/0,2).
Injection volume 20 l.
This work was supported by the Russian Foundation for
Basic Research (09-03-00519-).
*Corresponding author: elfimova_16@list.ru
Figure 1. Layout view of the adsorption of modified gold
nanoparticles on the silicon oxide’s surface.
[1] A. Vasil’kov, S. Nikolaev, V. Smirnov, A. Naumkin, I. Volkov
and V. Podshibikhin, Mendeleev Commun. 17, 268-270 (2007).
6th Nanoscience and Nanotechnology Conference, zmir, 2010 217