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Antimicrobials to Prevent Biodeterioration of Gra<strong>in</strong>s 95exhibited antifungal activity aga<strong>in</strong>st tested stra<strong>in</strong>s (Stojanovicet al., 2005).Organic solvent leaf extracts of two Moroccan Cistus L. species Cistusvillosus L. and Cistus monspeliensis L. (Cistaceae) used <strong>in</strong> traditional medic<strong>in</strong>ewere tested for their antifungal properties aga<strong>in</strong>st A. fumigatus. The extractsdiffered <strong>in</strong> their antifungal activities. C. villosus extracts exhibited higheractivity than C. monspeliensis (Bouamama et al., 2006).Antifungal activity of Trapa natans L. fruit r<strong>in</strong>d, extracted <strong>in</strong> different solventswith <strong>in</strong>creas<strong>in</strong>g polarity was observed and 1,4-dioxan, chloroform,acetone, dimethylformamide, ethanol, and water did not reveal any activityaga<strong>in</strong>st A. niger (Parekh and Chanda, 2007).4.3 In Vitro Evaluation of the Active Components for AntifungalActivity aga<strong>in</strong>st Important Biodeterioration-caus<strong>in</strong>g FungiPlants possess unlimited ability to produce secondary metabolites and morethan 12,000 of them have been isolated, which is probably less than 10% ofthe total. Useful antimicrobial phytochemicals are phenolics and poly phenols,qu<strong>in</strong>ones, flavones, flavonoids and flavanols, tann<strong>in</strong>s, coumar<strong>in</strong>s, terpenoidsand essential oils, alkaloids, and lect<strong>in</strong>s and polypeptides. Cowan(1999) has critically reviewed the antimicrobials of <strong>plant</strong> orig<strong>in</strong> and has listedthe <strong>plant</strong>s with antimicrobial activity.Many secondary metabolites have been identified and many more areyet to be discovered. There is grow<strong>in</strong>g evidence that most of these compoundsare <strong>in</strong>volved <strong>in</strong> the <strong>in</strong>teraction of <strong>plant</strong>s with other species, primarily<strong>in</strong> the defence of the <strong>plant</strong> from <strong>plant</strong> <strong>pest</strong>s. Secondary compounds representa large reservoir of chemical structures with biological activity (Duke, 1990).This resource is largely untapped for use as <strong>pest</strong>icides.Consider<strong>in</strong>g the advantages of organic <strong>pest</strong>icides over synthetic <strong>pest</strong>icides,nowadays, attempts are made by many workers to screen <strong>plant</strong>s forantimicrobial activity, isolate and characterize the bioactive compounds fromdifferent parts. Such attempts to isolate antimicrobials of <strong>plant</strong> orig<strong>in</strong> andtest their efficacy aga<strong>in</strong>st different storage fungi to prevent biodeteriorationof gra<strong>in</strong>s are, however, very few.The high terpene hydrocarbon content <strong>in</strong> the oils of chamomile, lavender,eucalyptus, and geranium are responsible for their antifungal activity aga<strong>in</strong>stA. niger and Aspergillus ochraceous (Lis-Balch<strong>in</strong> et al., 1998). Antimicrobialcompounds, such as 3-0-methylveracev<strong>in</strong>e, 3-0-[3-(2,2,2,-trifluoroethoxy)]-5-(methoxybenzoyl) veracev<strong>in</strong>e 3-0-(3,5-diiodobenzoyl) veracev<strong>in</strong>e, 3-0-(3-thienoyl) veracev<strong>in</strong>e, isolated from the seeds of Schoenocaulon offic<strong>in</strong>aleshowed highly significant antifungal activity aga<strong>in</strong>st A. flavus and A. niger(Oros and Ujvary, 1999).Mathekga et al. (2000) isolated an acylated form of a phlorogluc<strong>in</strong>ol fromHelichrysum caespititium. The structural elucidation revealed that the compoundwas 2-methyl-4-2’-4’(6’-trihydroxy-3’-(2-methylpropanoylphenyl)-2-0-enylacetate). This compound completely <strong>in</strong>hibited the mycelial growth

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