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252 M. Santos et al.teas coat <strong>plant</strong> surfaces (foliar application) or roots (liquid drench application)with liv<strong>in</strong>g microorganisms and provide food for beneficial microbes.Plasmopara viticola or downy mildew of grape leaves (Weltzien and Ketterer,1986), Botrytis c<strong>in</strong>erea or grey mould (Elad and Shtienberg, 1994; Diánez, 2005;Koné et al., 2010), Phytophthora c<strong>in</strong>namomi (Hoit<strong>in</strong>k et al., 1977), Fusariumoxysporum f.sp pisi or Fusarium wilt of peas (Khalifa, 1965), Fusarium oxysporumf.sp cucumer<strong>in</strong>um or Fusarium wilt of cucumber (Ma et al., 1999) Pythiumultimum or damp<strong>in</strong>g-off <strong>in</strong> pea (Pisum sativum) (Tränkner, 1992).Multiple modes of activity are <strong>in</strong>volved <strong>in</strong> suppress<strong>in</strong>g <strong>plant</strong> diseasewith NCT, whereas no studies have determ<strong>in</strong>ed the mechanism <strong>in</strong>volvedwith ACT (Scheuerell and Mahaffee, 2002a). The microbes <strong>in</strong> compost teascan suppress diseases <strong>in</strong> several ways: <strong>in</strong>duced resistance, antibiosis andcompetition (Br<strong>in</strong>ton, 1995; Scheuerell and Mahaffee, 2002a), and directdestruction of pathogens structures (Ma et al., 2001).The microbiotic of NCT (Weltzien, 1991) and ACT (Ingham, 2003) hadbeen described as be<strong>in</strong>g dom<strong>in</strong>ated by bacteria. It is important to know howthe manipulation of the compost tea production process enriches and/orselects for <strong>in</strong>dividual microbe populations. Scheuerell and Mahaffee (2002b)studied the use of ACT and NCT produced with and without nutrient additives,to drench peat-perlite grow<strong>in</strong>g media that was <strong>in</strong>oculated withPythium ultimum and <strong>plant</strong>ed with cucumber seeds. They used differentnutrient additives, fungal nutrients (soluble kelp, humic acids and rockdust) and bacterial nutrients (molasses-based nutrients solution). The mostconsistent compost tea formula for suppression damp<strong>in</strong>g-off <strong>in</strong> cucumberwas ACT produced with the fungal nutrients, whereas the disease was notsuppressed with ACT produced with the bacterial nutrients and withoutnutrients.12.4 Grape Marc CompostGrape marc is the waste from w<strong>in</strong>e production; once the juice has beenextracted, the sk<strong>in</strong> 7% (w/w), stalks 5% (w/w) and seeds 4% (w/w) are allredundant. In total, 15–20% of w<strong>in</strong>e production is waste, compris<strong>in</strong>g thousandsof tonnes. The amount of marc that is generated from fruit is known tovary for a number of reasons, <strong>in</strong>clud<strong>in</strong>g whether the grapes have been irrigatedand the type of equipment used to press the grapes (Jordan, 2002).Spanish production of grapes for w<strong>in</strong>e production is near 5 million tonnes;up to 700,000 tonnes of marc is estimated to have been generated throughoutSpa<strong>in</strong>. The marc, if not treated effectively, can cause a number of environmentalhazards rang<strong>in</strong>g from surface and groundwater pollution to foulsmells. In the European Union all by-<strong>products</strong> of w<strong>in</strong>e production (grapemarc and w<strong>in</strong>e lees) are obliged to be distilled; the legal basis for distillationmeasures <strong>in</strong> the EU is given <strong>in</strong> chapter II of title III, Art.27 of regulation (EC)N°1493/1999. In European countries grape marc is first distilled to recoveralcohol, followed by wash<strong>in</strong>g for tartrate recovery, then seed separation andf<strong>in</strong>ally the rema<strong>in</strong>der is either burnt for energy recovery or composted along

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