Control of Mycotox<strong>in</strong>s and Mycotoxigenic Fungi 37gas phase conta<strong>in</strong>ed at least 3.5 μg/ml, AITC was fungicidal to all testedfungi. Results of sensory evaluation showed that hot-dog bread was moresensitive to AITC than rye bread. The m<strong>in</strong>imal recognizable concentration ofAITC was 2.4 μg/ml gas phase for rye bread and between 1.8 and 3.5 μg/mlgas phase for hot-dog bread. These f<strong>in</strong>d<strong>in</strong>gs showed that the required shelflifeof rye bread could be achieved by AP with AITC. AP of hot-dog bread,may nevertheless require the additional effect of other preserv<strong>in</strong>g factors toavoid off-flavour formation (Nielsen and Rios, 2000).Comparisons between the effectiveness of the volatile gas phases and theliquid phases of essential oils have shown that oil <strong>in</strong> the liquid phase is moreeffective <strong>in</strong> prevent<strong>in</strong>g spoilage than when added via the gas phase. Highervolumes are required if the essential oils contact only the contam<strong>in</strong>at<strong>in</strong>gmicroorganisms <strong>in</strong> the gas phase. However, advantages of us<strong>in</strong>g a volatilegas phase of essential oil for food <strong>products</strong> are that it may have a lesser<strong>in</strong>fluence of the f<strong>in</strong>al taste and aroma of the product and its release may beregulated more easily.2.6 ConclusionsDur<strong>in</strong>g the past decade many publications have dealt with the <strong>in</strong>hibition ofmycotoxigenic species by <strong>natural</strong> <strong>plant</strong> <strong>products</strong>. Most of them showed ahigh efficacy of such <strong>products</strong> as antifungals. Their f<strong>in</strong>al application to food<strong>products</strong> is, however, still <strong>in</strong> its <strong>in</strong>fancy. Several reasons are <strong>in</strong>volved:●●●Different orig<strong>in</strong>, varieties and extraction methods of <strong>plant</strong> <strong>products</strong>result <strong>in</strong> essential oils and oleores<strong>in</strong>s that are widely varied <strong>in</strong> their composition.This heterogeneity prevents a direct extrapolation of results,unless experiments are carried out us<strong>in</strong>g pure components of theseessential oils and oleores<strong>in</strong>s.Plant <strong>products</strong> should be applied <strong>in</strong> such a way and at concentrationsthat do not affect gra<strong>in</strong> viability or the sensorial quality of food <strong>products</strong>.Most <strong>in</strong> vitro studies used high concentrations of <strong>plant</strong> extracts and useddirect contact as the screen<strong>in</strong>g technique, so the application of theseextracts to foods was not always successful.F<strong>in</strong>ally, safety issues should be fully addressed prior to the widespreadapplication of such <strong>plant</strong> <strong>products</strong>.ReferencesAdegoke, G.O. and Odesola, B.A. (1996) Storageof maize and cowpea and <strong>in</strong>hibitionof microbial agents of biodeteriorationus<strong>in</strong>g the powder and essential oil oflemon grass (Cymbopogon citratus). InternationalBiodeterioration & Biodegradation37, 81–84.Akpomeyade, D.E. and Ejechi, B.O. (1999)The hurdle effect of mild heat and twotropical spice extracts on the growth ofthree fungi <strong>in</strong> fruit juices. Food ResearchInternational 31, 339–341.Aldred, D., Cairns-Fuller, V. and Magan, N.(2008). Environmental factors affect
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